College of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Molecules. 2019 Oct 4;24(19):3576. doi: 10.3390/molecules24193576.
The chemical compositions of ethanol extracts of propolis from China (EEP-C) and the United States (EEP-A) and their antifungal activity against were determined. The result showed that a total of 49 compounds were detected by UPLC-Q-TOF-MS, 30 of which were present in samples from two regions. The major compounds of EEP-C and EEP-A were similar, including pinocembrin, pinobanksin-3--acetate, galanin, chrysin, pinobanksin, and pinobanksin-methyl ether, and both of them showed antifungal activity against with same minimum inhibitory concentration (MIC) value of 0.8 mg·mL. In the presence of propolis, the mycelial growth was inhibited, the hyphae became shriveled and wrinkled, the extracellular conductivities were increased, and the activities of succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) were decreased. In addition, iTRAQ-based quantitative proteomic analysis of in response to propolis revealed that a total of 341 proteins were differentially expressed, of which 88 (25.8%) were upregulated and 253 (74.2%) were downregulated. Meanwhile, the differentially expressed proteins (DEPs) involved in energy production and conversion, carbohydrate transport and metabolism, and the sterol biosynthetic pathway were identified. This study revealed that propolis could affect respiration, interfere with energy metabolism, and influence steroid biosynthesis to inhibit the growth of .
测定了中国（EEP-C）和美国（EEP-A）蜂胶乙醇提取物的化学成分及其对的抗真菌活性。结果表明，通过 UPLC-Q-TOF-MS 共检测到 49 种化合物，其中 30 种存在于两个地区的样品中。EEP-C 和 EEP-A 的主要化合物相似，包括 pinocembrenin、pinobanksin-3--acetate、galanin、chrysin、pinobanksin 和 pinobanksin-methyl ether，它们对均表现出相同的最低抑菌浓度（MIC）值为 0.8 mg·mL 的抗真菌活性。在蜂胶存在的情况下，菌丝生长受到抑制，菌丝变得干瘪起皱，细胞外电导率增加，琥珀酸脱氢酶（SDH）和苹果酸脱氢酶（MDH）的活性降低。此外，对响应蜂胶的的 iTRAQ 定量蛋白质组学分析表明，共有 341 种蛋白质表达差异，其中 88 种（25.8%）上调，253 种（74.2%）下调。同时，确定了参与能量产生和转化、碳水化合物运输和代谢以及甾体生物合成途径的差异表达蛋白（DEPs）。本研究表明，蜂胶可以影响呼吸，干扰能量代谢，影响甾体生物合成，从而抑制的生长。
