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[125I]脱氧胞苷用于一种快速、灵敏且特异的1型单纯疱疹病毒胸苷激酶检测方法。

[125I]deoxycytidine used in a rapid, sensitive, and specific assay for herpes simplex virus type 1 thymidine kinase.

作者信息

Summers W C, Summers W P

出版信息

J Virol. 1977 Oct;24(1):314-8. doi: 10.1128/JVI.24.1.314-318.1977.

DOI:10.1128/JVI.24.1.314-318.1977
PMID:198581
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC515932/
Abstract

[125I]deoxycytidine was a good substrate for herpes simplex virus type 1 thymidine kinase (TK), whereas [125I]deoxycytidine was a very poor substrate for cellular TK. Simple, sensitive, and specific assays for viral TK could be carried out in vivo and in vitro even in the presence of cell TK. Autoradiographic detection of incorporated [125I]deoxycytidine provided a rapid and simple method for detection of and screening for viral TK in infected as well as viral TK-transformed cells.

摘要

[125I]脱氧胞苷是单纯疱疹病毒1型胸苷激酶（TK）的良好底物，而[125I]脱氧胞苷是细胞TK的非常差的底物。即使存在细胞TK，也可以在体内和体外进行简单、灵敏且特异的病毒TK检测。对掺入的[125I]脱氧胞苷进行放射自显影检测，为在感染细胞以及病毒TK转化细胞中检测和筛选病毒TK提供了一种快速且简单的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/821f/515932/7dac84a62761/jvirol00214-0330-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/821f/515932/7dac84a62761/jvirol00214-0330-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/821f/515932/7dac84a62761/jvirol00214-0330-a.jpg

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[125I]deoxycytidine used in a rapid, sensitive, and specific assay for herpes simplex virus type 1 thymidine kinase.[125I]脱氧胞苷用于一种快速、灵敏且特异的1型单纯疱疹病毒胸苷激酶检测方法。
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MUTANT STRAINS OF HERPES SIMPLEX DEFICIENT IN THYMIDINE KINASE-INDUCING ACTIVITY.胸苷激酶诱导活性缺陷的单纯疱疹突变株。
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Iodination of nucleic acids in vitro.核酸的体外碘化
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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
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Thymidine plaque autoradiography of thymidine kinase-positive and thymidine kinase-negative herpesviruses.胸苷激酶阳性和胸苷激酶阴性疱疹病毒的胸苷斑块放射自显影术。
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Functional suppression in mammalian cells of nonsense mutations in the herpes simplex virus thymidine kinase gene by suppressor tRNA genes.通过抑制性tRNA基因对单纯疱疹病毒胸苷激酶基因无义突变在哺乳动物细胞中的功能抑制
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