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胸苷激酶阳性和胸苷激酶阴性疱疹病毒的胸苷斑块放射自显影术。

Thymidine plaque autoradiography of thymidine kinase-positive and thymidine kinase-negative herpesviruses.

作者信息

Tenser R B, Jones J C, Ressel S J, Fralish F A

出版信息

J Clin Microbiol. 1983 Jan;17(1):122-7. doi: 10.1128/jcm.17.1.122-127.1983.

DOI:10.1128/jcm.17.1.122-127.1983
PMID:6826696
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272586/
Abstract

Plaques formed by herpes simplex virus (HSV), pseudorabies virus, and varicella-zoster virus were studied by plaque autoradiography after [14C]thymidine labeling. Standard thymidine kinase-positive (TK+) viruses and TK- mutants of HSV types 1 and 2 and pseudorabies virus were studied, including cell cultured viruses and viruses isolated from animals. Autoradiography was performed with X-ray film with an exposure time of 5 days. After development of films, TK+ plaques showed dark rims due to isotope incorporation, whereas TK- plaques were minimally labeled. Plaque autoradiography of stock TK- viruses showed reversion frequencies to the TK+ phenotype of less than 10(-3). Autoradiography indicated that TK- virus retained the TK- phenotype after replication in vivo. In addition, it was shown that TK- HSV could be isolated from mouse trigeminal ganglion tissue after corneal inoculation of TK- HSV together with TK+ HSV. The plaque autoradiographic procedure was very useful to evaluate proportions of TK+ and TK- virus present in TK+-TK- virus mixtures.

摘要

在[14C]胸苷标记后，通过噬斑放射自显影术研究了由单纯疱疹病毒（HSV）、伪狂犬病病毒和水痘-带状疱疹病毒形成的噬斑。研究了1型和2型HSV以及伪狂犬病病毒的标准胸苷激酶阳性（TK+）病毒和TK-突变体，包括细胞培养病毒和从动物体内分离的病毒。使用X射线胶片进行放射自显影，曝光时间为5天。胶片显影后，由于同位素掺入，TK+噬斑显示出深色边缘，而TK-噬斑标记极少。库存TK-病毒的噬斑放射自显影显示，其向TK+表型的回复频率低于10（-3）。放射自显影表明，TK-病毒在体内复制后仍保留TK-表型。此外，研究表明，在将TK- HSV与TK+ HSV一起角膜接种后，可从小鼠三叉神经节组织中分离出TK- HSV。噬斑放射自显影程序对于评估TK+-TK-病毒混合物中TK+和TK-病毒的比例非常有用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/b386b18c89c1/jcm00138-0153-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/f227acba0b55/jcm00138-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/6c58f0a79f43/jcm00138-0151-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/87cdeb7c6c5b/jcm00138-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/2b5d85b16ace/jcm00138-0152-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/bcf83a7648ce/jcm00138-0153-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/b386b18c89c1/jcm00138-0153-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/f227acba0b55/jcm00138-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/6c58f0a79f43/jcm00138-0151-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/87cdeb7c6c5b/jcm00138-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/2b5d85b16ace/jcm00138-0152-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/bcf83a7648ce/jcm00138-0153-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8138/272586/b386b18c89c1/jcm00138-0153-b.jpg

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