Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.
IUBMB Life. 2009 Nov;61(11):1075-82. doi: 10.1002/iub.252.
MicroRNAs are a group of endogenously expressed, single-stranded, 18-24 nt RNAs that regulate diverse cellular pathways. Although documented evidence indicates that some microRNAs can function as oncogenes or tumor-suppressors, the role of miR-214 in regulating human cervical cancer cells remains unexplored. We determined the expression level of miR-214 and found it is downregulated in cervical cancer compared with normal tissue. Overexpression of miR-214 in HeLa cells, a human cervical cancer cell line, significantly inhibited cell proliferation according to the MTT and colony forming assays. HeLa cells that stably overexpress miR-214 downregulate the expression of MEK3 and JNK1 at both mRNA and protein levels. Further investigation revealed that miR-214 regulates the expression of MEK3 and JNK1 by targeting the 3'UTRs of these genes. Collectively, these results suggest that miR-214 negatively regulates HeLa cell proliferation by targeting the noncoding regions of MEK3 and JNK1 mRNAs.
微小 RNA 是一组内源性表达的、单链的、18-24 个核苷酸的 RNA，调节多种细胞途径。尽管有文献证据表明，一些微小 RNA 可以作为癌基因或肿瘤抑制因子发挥作用，但 miR-214 在调节人宫颈癌细胞中的作用仍未被探索。我们测定了 miR-214 的表达水平，发现其在宫颈癌组织中较正常组织下调。在人宫颈癌细胞系 HeLa 细胞中过表达 miR-214，根据 MTT 和集落形成测定，可显著抑制细胞增殖。稳定过表达 miR-214 的 HeLa 细胞下调 MEK3 和 JNK1 的 mRNA 和蛋白水平表达。进一步的研究表明，miR-214 通过靶向这些基因的 3'UTRs 来调节 MEK3 和 JNK1 的表达。总之，这些结果表明，miR-214 通过靶向 MEK3 和 JNK1 mRNA 的非编码区负调控 HeLa 细胞的增殖。
