Musialkivs'ka A O, Pozdniakova T M, Platonova T M, Khomenko V M
Ukr Biokhim Zh. 1977 Jul-Aug;49(4):118-23.
A method is developed for the quantitative determination of the protector activity. The method is based on registrating the coagulation time shortening for a mixture of fibrin monomer and fragment D. All determinations should be carried out at high concentrations of fragment D when its inhibitory effect on fibrin polymerization is very strong. It is necessary to control the exact conditions as to ionic strength and pH of the reaction mixture. Another approach to the protector activity estimation is demonstrated. It is based on determination of clot protein quantities after fibrin monomer clotting in mixtures with constant fragment D and different protector quantities.
开发了一种用于定量测定保护剂活性的方法。该方法基于记录纤维蛋白单体和D片段混合物的凝血时间缩短情况。所有测定应在D片段浓度较高时进行，此时其对纤维蛋白聚合的抑制作用非常强。必须控制反应混合物离子强度和pH的精确条件。展示了另一种评估保护剂活性的方法。它基于在含有恒定D片段和不同保护剂数量的混合物中，纤维蛋白单体凝血后测定凝块蛋白量。
