Uharova T P, Smekhova K O, Musjalkivs'ka A O
Ukr Biokhim Zh. 1977 Sep-Oct;49(5):60-3.
The interaction was studied between two fragments of bovine fibrinogen isolated from its tryptic hydrolysate: the D fragment, an inhibitor of fibrin-monomer polymerization, and the protector, a fragment inactivating the D fragment. This reaction is relatively rapid: it is completed for several minutes at room temperature and at 37 degrees and is slowed down at 5 degrees. However, the reached level of the D fragment inactivation does not depend upon temperature (within a range from 5 degrees to 37 degrees). Dilution of the incubation mixture under applied conditions caused no dissociation of the D fragment-protector complex. Inactivation of the D fragment by the protector depends on the solution ionic strength; ionic strength 0.13-0.16 is optimal.
D片段，一种纤维蛋白单体聚合的抑制剂，以及保护剂，一种使D片段失活的片段。该反应相对较快：在室温及37摄氏度下几分钟内即可完成，在5摄氏度时反应减慢。然而，D片段失活所达到的水平并不取决于温度（在5摄氏度至37摄氏度范围内）。在所应用的条件下孵育混合物的稀释不会导致D片段 - 保护剂复合物的解离。保护剂使D片段失活取决于溶液的离子强度；离子强度0.13 - 0.16是最佳的。
