Department of Gastroenterology, 456 Hospital of PLA, 25 Wuyingshan Road, Jinan 250031, Shandong, China.
Food Chem Toxicol. 2010 Jan;48(1):390-5. doi: 10.1016/j.fct.2009.10.027. Epub 2009 Oct 25.
The chemopreventive effects of curcumin and green tea catechins individually and in combination on 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis were studied in male Wister rats following 32 weeks of dietary treatment. The incidence, number and size of colorectal cancer were measured. Colorectal aberrant crypt foci (ACF) were analyzed by methylene blue staining. Proliferation indices and apoptotic indices were determined by PCNA immunostaining and TUNEL assay, respectively. The results showed that dietary curcumin, catechins and combination administration significantly inhibited the total number of ACF per rat. The combination treatment displayed the most potent inhibitory effect, while there was no difference of inhibition between curcumin and catechins-treated groups. The incidence of colorectal cancer in the treated groups was significantly lower than that of positive control group. Compared with the positive control group, the proliferation index was significantly decreased and the apoptotic index was significantly increased in all treatment groups, while the effect of the combination was the greatest among the treated groups. Our findings suggest that the combination of curcumin and catechins may produce a synergistic colon cancer-preventative effect that would be more potent than each of the compounds alone.
研究了姜黄素和绿茶儿茶素单独及联合应用对 1,2-二甲基肼（DMH）诱导的雄性 Wistar 大鼠结肠癌发生的化学预防作用，在膳食处理 32 周后进行。测量了结直肠肿瘤的发生率、数量和大小。采用亚甲蓝染色分析结直肠异型隐窝病灶（ACF）。通过 PCNA 免疫染色和 TUNEL 测定分别测定增殖指数和凋亡指数。结果表明，膳食姜黄素、儿茶素和联合给药显著抑制每只大鼠的 ACF 总数。联合治疗显示出最强的抑制作用，而姜黄素和儿茶素治疗组之间没有差异。与阳性对照组相比，所有治疗组的结直肠癌发生率均显著降低。与阳性对照组相比，所有治疗组的增殖指数均显著降低，凋亡指数均显著升高，而联合治疗组的效果最大。我们的研究结果表明，姜黄素和儿茶素的联合应用可能产生协同的结肠癌预防作用，比单独使用任何一种化合物都更有效。
