Madrigal-Bujaidar E, Sánchez-Sánchez M A
Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, I.P.N. Carpio y Plan de Ayala Col. Santo Tomás, Mexico D.F. Mexico.
Mutat Res. 1991 Jan;262(1):15-9. doi: 10.1016/0165-7992(91)90099-p.
The quality of sister-chromatid differentiation, the basal rate of sister-chromatid exchanges (SCE) and the rate of cellular proliferation were studied in untreated and mitomycin C(MMC)-treated mice, using 4 different systems for administering 5-bromodeoxyuridine (BrdU): BrdU adsorbed to charcoal, tablets of BrdU mixed with cholesterol, tablets of BrdU coated with agar and tablets of BrdU partially coated with paraffin. The quality of sister-chromatid differentiation with the studied methods showed a useful stain contrast in an average of 75.4% second-division mitosas, with the lowest average occurring in mice implanted with agar-coated tablets. The frequency of SCE and the replicative index were similar in mice administered BrdU by all 4 systems both in control and in MMC-treated mice. From a practical point of view, the charcoal method could be done the fastest.
使用4种不同的5-溴脱氧尿苷（BrdU）给药系统，即吸附于活性炭上的BrdU、与胆固醇混合的BrdU片剂、琼脂包被的BrdU片剂以及部分用石蜡包被的BrdU片剂，研究了未处理和丝裂霉素C（MMC）处理的小鼠中姐妹染色单体分化质量、姐妹染色单体交换（SCE）的基础率和细胞增殖率。所研究方法的姐妹染色单体分化质量在平均75.4%的第二次有丝分裂中显示出有用的染色对比，平均最低值出现在植入琼脂包被片剂的小鼠中。在对照小鼠和MMC处理的小鼠中，通过所有4种系统给予BrdU的小鼠中SCE频率和复制指数相似。从实际角度来看，活性炭法操作最快。
