Department of Otorhinolaryngology, Medical University of Vienna, Waehringer Gürtel 18-20, 1090 Vienna, Austria.
Department of Cranio-Maxillofacial and Oral Surgery, Medical University of Vienna, Waehringer Gürtel 18-20, 1090 Vienna, Austria.
Biomed Res Int. 2019 Jul 9;2019:8051270. doi: 10.1155/2019/8051270. eCollection 2019.
Microbial colonization of silicone voice prostheses by bacteria and species limits the device lifetime of modern voice prostheses in laryngectomized patients. Thus, research focuses on biofilm inhibitive properties of novel materials, coatings, and surface enhancements. Goal of this study was the evaluation of seven commonly used growth media to simulate growth of mixed oropharyngeal species as mesoscale biofilms on prosthetic silicone for future research purposes.
Yeast Peptone Dextrose medium (YPD), Yeast Nitrogen Base medium (YNB), M199 medium, Spider medium, RPMI 1640 medium, Tryptic Soy Broth (TSB), and Fetal Bovine Serum (FBS) were used to culture combined mixed strains and mixed bacterial-fungal compositions on silicone over the period of 22 days. The biofilm surface spread and the microscopic growth showed variations from biofilms depending on the microbial composition and growth medium.
YPD and FBS prove to support continuous growth of mixed bacterial-fungal oropharyngeal biofilms deposits over weeks as needed for longterm testing with oropharyngeal biofilm compositions.
The study provides data on culture conditions for mixed multispecies biofilm compositions that can be used for future prosthesis designs.
细菌对硅胶语音假体的微生物定植限制了喉切除患者现代语音假体的使用寿命。因此，研究集中在新型材料、涂层和表面增强的生物膜抑制特性上。本研究的目的是评估七种常用的生长培养基，以模拟混合口咽物种在假体硅胶上作为中尺度生物膜的生长，用于未来的研究目的。
使用酵母肽葡萄糖培养基（YPD）、酵母氮基培养基（YNB）、M199 培养基、蜘蛛培养基、RPMI 1640 培养基、胰蛋白酶大豆肉汤（TSB）和胎牛血清（FBS）培养混合混合菌株和混合细菌-真菌组成在硅胶上 22 天。生物膜表面扩散和微观生长显示出依赖于微生物组成和生长培养基的差异。
YPD 和 FBS 被证明可以支持混合细菌-真菌口咽生物膜沉积物的连续生长，这是口咽生物膜组成物进行长期测试所需的。
本研究提供了用于未来假体设计的混合多物种生物膜组成物的培养条件数据。
