Zabłocka-Godlewska Ewa, Przystaś Wioletta, Grabińska-Sota Elżbieta
Environmental Biotechnology Department, Silesian University of Technology, Akademicka 2A, 44-100 Gliwice, Poland.
Water Air Soil Pollut. 2015;226(1):2249. doi: 10.1007/s11270-014-2249-6. Epub 2014 Dec 11.
This study aimed to decolourise different dyes using two strains (Bz4 and Rz7) in different concentrations and incubation conditions. Azo (Evans blue (EB)) and triphenylmethane (brilliant green (BG)) dyes were used individually and in mixture. The toxicity of the biotransformation products was estimated. Both strains had a significant potential to decolourise the dyes in the fluorone, azo and triphenylmethane classes. The type and concentration of dye affects the decolourisation effectiveness. Differences in the dye removal potential were observed particularly in the main experiment. The best results were obtained for Bz4 in the samples with EB (up to 95.4 %) and dye mixture (up to 99 %) and for Rz7 with BG (100 %). The living and dead biomass of the strain Bz4 highly absorbs the dyes. Significant effect of the process conditions was noticed for both strains. The best results were obtained in static and semistatic samples (89-99 %) for the removal of EB and a mixture of dyes and in static samples (100 %) for BG. The decrease in zootoxicity (from class IV/V) was noticed in all samples with living biomass of the strain Bz4 (to class III/IV) and in samples with single dyes for Rz7 (to class III/IV). The decrease in phytotoxicity (from class III/IV) was noticed for Bz4 in the samples with BG and a mixture (to class III) and for Rz7 in the samples with BG (to class III). The process conditions did not affect the changes in toxicity after the process.
本研究旨在使用两种菌株（Bz4和Rz7）在不同浓度和培养条件下对不同染料进行脱色。单独使用和混合使用了偶氮染料（伊文思蓝（EB））和三苯甲烷染料（亮绿（BG））。评估了生物转化产物的毒性。两种菌株都具有显著的潜力对芴、偶氮和三苯甲烷类染料进行脱色。染料的类型和浓度会影响脱色效果。在主要实验中尤其观察到了染料去除潜力的差异。对于含有EB的样品（高达95.4%）和染料混合物（高达99%）中的Bz4以及含有BG的样品（100%）中的Rz7，获得了最佳结果。菌株Bz4的活生物质和死生物质对染料有高度吸收。注意到工艺条件对两种菌株都有显著影响。对于去除EB和染料混合物，在静态和半静态样品中（89 - 99%）获得了最佳结果，对于BG，在静态样品中（100%）获得了最佳结果。在所有含有菌株Bz4活生物质的样品中（降至III/IV级）以及含有Rz7单一染料的样品中（降至III/IV级），观察到动物毒性降低（从IV/V级）。对于含有BG和混合物的样品中的Bz4（降至III级）以及含有BG的样品中的Rz7（降至III级），观察到植物毒性降低（从III/IV级）。工艺条件不影响工艺后毒性的变化。
