多重 PCR-连接酶检测反应法用于同时检测金黄色葡萄球菌、粪肠球菌和屎肠球菌的耐药性和毒素基因。
Multiplex PCR-ligation detection reaction assay for simultaneous detection of drug resistance and toxin genes from Staphylococcus aureus, Enterococcus faecalis, and Enterococcus faecium.
机构信息
Department of Microbiology, Division of Infectious Diseases, Weill Medical College of Cornell University, New York, New York, USA.
出版信息
J Clin Microbiol. 2010 Jan;48(1):277-80. doi: 10.1128/JCM.01411-09. Epub 2009 Oct 28.
Abstract
A multiplex PCR-ligation detection reaction (PCR-LDR) assay was developed for rapid detection of methicillin, tetracycline, and vancomycin resistance, as well as toxic shock toxin and Panton-Valentine leukocidin. The assay was tested on 470 positive blood culture bottles containing Staphylococcus aureus or enterococci. PCR-LDR exhibited a sensitivity and specificity of > or = 98% for all components except tetracycline resistance, which had a sensitivity of 94.7%. Rapid and sensitive detection of antimicrobial resistance and virulence genes could help guide therapy and appropriate infection control measures.
摘要
建立了一种多重聚合酶链反应-连接酶检测反应（PCR-LDR）检测方法，用于快速检测耐甲氧西林、四环素和万古霉素，以及中毒性休克综合征毒素和杀白细胞素。该方法在 470 个含有金黄色葡萄球菌或肠球菌的阳性血培养瓶上进行了测试。PCR-LDR 对所有成分的敏感性和特异性均>或=98%，除了四环素耐药性的敏感性为 94.7%。快速、敏感地检测抗菌药物耐药性和毒力基因有助于指导治疗和采取适当的感染控制措施。
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