Di Meo G P, Perucatti A, Peretti V, Incarnato D, Ciotola F, Liotta L, Raudsepp T, Di Berardino D, Chowdhary B, Iannuzzi L
National Research Council (CNR), ISPAAM, Laboratory of Animal Cytogenetics and Gene Mapping, Naples, Italy.
Cytogenet Genome Res. 2009;125(4):266-71. doi: 10.1159/000235932. Epub 2009 Oct 27.
Donkey chromosomes were earlier characterized separately by C-, G- and R-banding techniques. However, direct comparisons between G- and R-banding patterns have still not been carried out in this species. The present study reports this comparison at the 450-band level by using replication G- and R-banding patterns. Two sets of synchronized lymphocyte cultures were set up to obtain early (GBA+CBA-banding) and late (RBA-banding) BrdU incorporation. Slides were stained with acridine orange and observed under a fluorescence microscope. Reverse GBA+CBA- and RBA-banded karyotypes at the 450-band level were constructed. To verify G- and R-banding patterns in some acrocentric chromosomes, sequential GBA+CBA/Ag-NORs and RBA/Ag-NORs were also performed. The results of CBA-banding patterns obtained in 12 animals from 2 breeds showed a pronounced polymorphism of heterochromatin, especially in EAS1q-prox. Ideogrammatic representations of G- and R-banded karyotypes were constructed using only one common G- and R-banding nomenclature. In the present study both G- and R-banding patterns and relative ideograms are presented as standard karyotype for this species at the 450-band level.
驴的染色体早期是通过C带、G带和R带技术分别进行特征描述的。然而，该物种尚未对G带和R带模式进行直接比较。本研究通过使用复制G带和R带模式，报告了在450条带水平上的这种比较。建立了两组同步淋巴细胞培养物，以获得早期（GBA+CBA带）和晚期（RBA带）的BrdU掺入。玻片用吖啶橙染色并在荧光显微镜下观察。构建了450条带水平的反向GBA+CBA和RBA带核型。为了验证一些近端着丝粒染色体中的G带和R带模式，还进行了连续的GBA+CBA/Ag-NORs和RBA/Ag-NORs实验。从2个品种的12只动物中获得的CBA带模式结果显示，异染色质存在明显的多态性，尤其是在EAS1q-prox区域。仅使用一种通用的G带和R带命名法构建了G带和R带核型的模式图。在本研究中，G带和R带模式以及相关模式图均作为该物种在450条带水平的标准核型呈现。
