Department of Pediatrics, Guangdong Women and Children's Hospital, Guangzhou, China.
Med Sci Monit. 2011 Aug;17(8):CR423-431. doi: 10.12659/msm.881903.
Human cytomegalovirus (HCMV) is a leading cause of morbidity and mortality in immunocompromised individuals. The unique long b' (ULB') region of HCMV contains at least 19 open reading frames (ORFs); however, little is known about the function of UL145 and UL136. We characterized UL145 and UL136 in low-passage clinical isolates from Chinese infants.
MATERIAL/METHODS: The clinical strains of HCMV were recovered from the urine from HCMV-infected infants. Human embryonic lung fibroblasts (HELFs) were infected with clinical isolates of HCMV, and the viral DNA and mRNA for UL145 and UL136 were analyzed by polymerase chain reaction (PCR) and sequencing techniques. We also predicted the structure and function of UL145 and UL136 proteins.
Sixty-two Chinese infants infected with HCMV were recruited into this study and the clinical isolates were recovered from the urine. Two strains among the low-passage isolates, D2 and D3, were obtained. The UL145 and UL136 sequences were deposited with GenBank under accession numbers of DQ180367, DQ180381, DQ180377, and DQ180389. The mRNA expression of both UL145 and UL136 was confirmed by reverse transcription (RT-PCR) assays. UL145 was predicted to contain 1 protein kinase C phosphorylation site, 2 casein kinase II phosphorylation sites and a zinc finger structure. UL136 was predicted to contain a protein kinase C phosphorylation site, N-myristoylation site, cAMP- and cGMP-dependent protein kinase phosphorylation site and tyrosine kinase II phosphorylation site. Both UL145 and UL136 are highly conserved.
UL145 may act as an intranuclear regulating factor by direct binding to DNA, while UL136 may be a membrane receptor involving signal transduction.
人巨细胞病毒（HCMV）是免疫功能低下个体发病和死亡的主要原因。HCMV 的独特长 b'（ULB'）区域包含至少 19 个开放阅读框（ORF）；然而，人们对 UL145 和 UL136 的功能知之甚少。我们对来自中国婴儿的低传代临床分离株中的 UL145 和 UL136 进行了特征描述。
材料/方法：HCMV 的临床分离株是从 HCMV 感染婴儿的尿液中回收的。用人胚胎肺成纤维细胞（HELF）感染 HCMV 的临床分离株，通过聚合酶链反应（PCR）和测序技术分析 UL145 和 UL136 的病毒 DNA 和 mRNA。我们还预测了 UL145 和 UL136 蛋白的结构和功能。
本研究共招募了 62 名感染 HCMV 的中国婴儿，从尿液中回收了临床分离株。从低传代分离株中获得了 2 株，即 D2 和 D3。UL145 和 UL136 序列已在 GenBank 中注册，登录号为 DQ180367、DQ180381、DQ180377 和 DQ180389。通过反转录（RT-PCR）试验证实了 UL145 和 UL136 的 mRNA 表达。预测 UL145 包含 1 个蛋白激酶 C 磷酸化位点、2 个酪蛋白激酶 II 磷酸化位点和一个锌指结构。预测 UL136 包含一个蛋白激酶 C 磷酸化位点、N-豆蔻酰化位点、cAMP 和 cGMP 依赖性蛋白激酶磷酸化位点和酪氨酸激酶 II 磷酸化位点。UL145 和 UL136 均高度保守。
UL145 可能通过直接与 DNA 结合作为核内调节因子发挥作用，而 UL136 可能是一种参与信号转导的膜受体。
