Departments of Pathology, Harvard Medical School, and the Boston Lying-In Hospital, Boston.
J Exp Med. 1962 May 1;115(5):977-96. doi: 10.1084/jem.115.5.977.
The H antigen was mapped out by immunofluorescence in human tissues (including those of fetuses from 15 cm crown-heel length) from individuals of the various groups within the ABO system, both secretors and non-secretors. The distribution of the antigen can be summarized under the following headings: Cell walls of endothelium: present throughout the cardiovascular system; Cell walls of stratified epithelia: in skin, non-cornifying squamous stratified membranes, transitional epithelia; Mucus: occurring wherever the latter is produced in secretor individuals and confined to a few special topographical areas in non-secretors; Secretions and excretions: the pancreatic and sudoriferous (independent of secretor status), and mammary and uterine (governed by the secretor makeup) all contain it. The distribution of the H antigen is most fully represented in tissues of group O. It follows an over-all universal pattern, characteristically modified in non-secretors, equally valid for antigens A and B described in a preceding study. Within this pattern, in tissues of the non-O groups, the complement of the H substance in its various forms wanes in a manner consistent with the hypothesis that it serves as a substrate for the A(1), A(2), B genes, exerting their action with different degrees of efficiency. The secretor:non-secretor phenomena can be most simply interpreted by viewing the non-secretor, recessive gene (in the homozygous, ss condition) as inhibiting the production of some of the water-soluble forms of the blood group substances. Since the gene was never found responsible for dissociation of the H and A, B antigens its inhibitory action is thought to be wrought at the point of formation of the basic H substance or its precursor.
H 抗原在人类组织（包括 15 厘米冠臀长的胎儿组织）中通过免疫荧光技术进行了定位，这些组织来自 ABO 系统各个群体的个体，包括分泌型和非分泌型个体。抗原的分布可以概括为以下几个方面：内皮细胞细胞壁：存在于心血管系统的各个部位；角化鳞状分层上皮细胞壁：在皮肤、非角化的鳞状分层膜、过渡上皮中；粘液：在分泌型个体中产生粘液的地方都存在，而在非分泌型个体中仅局限于少数特殊的局部区域；分泌和排泄：胰腺和汗腺（与分泌状态无关），以及乳腺和子宫（受分泌成分控制）都含有 H 抗原。H 抗原在 O 型血组织中的分布最为完整。它遵循一个整体的普遍模式，在非分泌型个体中特征性地发生改变，与之前研究中描述的 A 抗原和 B 抗原的模式完全一致。在这个模式中，在非 O 型血组织中，H 物质的各种形式的补充量逐渐减少，这与它作为 A(1)、A(2)、B 基因的底物的假设一致，其作用的效率不同。分泌型和非分泌型现象可以通过将非分泌型、隐性基因（在纯合子 ss 条件下）视为抑制某些血型物质的水溶性形式的产生来最简化地解释。由于该基因从未被发现负责 H 和 A、B 抗原的分离，因此其抑制作用被认为是在基本 H 物质或其前体形成的点上发挥作用的。
