不同文化介质特性及其对器官外组织生长影响的研究。
STUDIES UPON THE CHARACTERISTICS OF DIFFERENT CULTURE MEDIA AND THEIR INFLUENCE UPON THE GROWTH OF TISSUE OUTSIDE OF THE ORGANISM.
机构信息
Laboratories of The Rockefeller Institute for Medical Research, New York.
出版信息
J Exp Med. 1912 Oct 1;16(4):421-31. doi: 10.1084/jem.16.4.421.
Abstract
- There is a great difference between embryonic and adult tissue as far as their growth outside of the organism is concerned. Adult tissue grows only in plasma. Embryonic tissue grows also very well in serum and serum plus agar. In Ringer's solution and in Ringer's solution plus agar no growth occurs, whether embryonic or adult tissue is employed; survival and emigration of cells are seen to some extent. 2. For the growth of connective tissue cells of chick embryo, unheated homogenic serum is a better culture medium than heated serum. The growth of epithelial cells is not thus influenced. 3. Heated heterogenic serum is a better culture medium for growth of embryonic connective tissue cells than unheated. 4. There is an inverse ratio between the hemolytic power of heterogenic sera and the extent of growth of tissue in them. This inverse ratio is not found in heterogenic plasmas.
摘要
就组织在机体外的生长而言，胚胎组织和成年组织之间存在很大差异。成年组织仅在血浆中生长。胚胎组织在血清和血清加琼脂中也能很好地生长。在林格氏溶液和林格氏溶液加琼脂中，无论是胚胎组织还是成年组织，都不会发生生长；在某种程度上可以看到细胞的存活和迁移。
对于鸡胚结缔组织细胞的生长，未加热的同源血清是比加热血清更好的培养基。上皮细胞的生长不受影响。
加热的异种血清是比未加热的更适合胚胎结缔组织细胞生长的培养基。
异种血清的溶血能力与组织在其中的生长程度之间呈反比关系。这种反比关系在异种血浆中不存在。
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