Ambrose Charles T
University of Kentucky College of Medicine, Lexington, KY, USA.
In Vitro Cell Dev Biol Anim. 2017 May;53(5):467-473. doi: 10.1007/s11626-016-0128-8. Epub 2017 Jan 11.
George Otto Gey was a pioneer in tissue culture, having introduced the roller drum, the HeLa cell line, and the use of human fetal cord serum and beef embryo extract. During his career (1920s-1960s), the field of tissue culture was in its infancy and not yet dependent upon commercial biological supply houses. While the early techniques of cell culture have been greatly improved upon, of historical interest may be personal observations of the Gey Tissue Culture Laboratory, Johns Hopkins Medical School, as recalled by a medical student working there in the 1950s. Dr. Gey served as a founding member and executive of the Tissue Culture Commission (TCC) and became the first president of the Tissue Culture Association (TCA).
乔治·奥托·盖伊是组织培养领域的先驱,他引入了滚筒、海拉细胞系,以及人胎脐带血清和牛胚胎提取物的使用。在他的职业生涯(20世纪20年代至60年代)中,组织培养领域尚处于起步阶段,尚未依赖商业生物供应公司。虽然细胞培养的早期技术已经有了很大改进,但20世纪50年代在约翰霍普金斯医学院盖伊组织培养实验室工作的一名医科学生回忆起的该实验室的个人观察结果可能具有历史意义。盖伊博士是组织培养委员会(TCC)的创始成员和管理人员,并成为组织培养协会(TCA)的第一任主席。