Robbins K T, Varki N M, Storniolo A M, Hoffman H, Hoffman R M
Division of Otolaryngology-Head and Neck Surgery, University of California, School of Medicine, La Jolla.
Arch Otolaryngol Head Neck Surg. 1991 Jan;117(1):83-6. doi: 10.1001/archotol.1991.01870130089022.
We describe a chemosensitivity testing of head and neck tumors, in which a native-state histoculture, ie, a three-dimensional culture system that maintains important in vivo properties, including tissue architecture, was used. Fifteen specimens of head and neck tumors were evaluated for sensitivity to the following drugs: cisplatin (DDP) at concentrations of 1.5, 15, and 37.5 micrograms/mL; fluorouracil at concentrations of 4.0, 40, and 100 micrograms/mL; and combinations of cisplatin and fluorouracil in corresponding doses. Growth and measurement of drug responses were successfully completed in 10 specimens (five others were contaminated, four of them prior to instituting rigorous antibiotic washes). The results indicated cisplatin sensitivity in five of 10 patients; fluorouracil sensitivity in four of 10 patients; and fluorouracil-cisplatin sensitivity in seven of eight patients. Our preliminary results indicate that the native-state histoculture technique is feasible to test chemosensitivity of head and neck tumors.
我们描述了一种头颈部肿瘤的化学敏感性测试，其中使用了一种天然状态的组织培养，即一种维持包括组织结构在内的重要体内特性的三维培养系统。对15例头颈部肿瘤标本进行了对以下药物的敏感性评估：浓度为1.5、15和37.5微克/毫升的顺铂（DDP）；浓度为4.0、40和100微克/毫升的氟尿嘧啶；以及相应剂量的顺铂和氟尿嘧啶组合。在10个标本中成功完成了药物反应的生长和测量（另外5个被污染，其中4个在进行严格的抗生素冲洗之前就已被污染）。结果表明，10例患者中有5例对顺铂敏感；10例患者中有4例对氟尿嘧啶敏感；8例患者中有7例对氟尿嘧啶-顺铂敏感。我们的初步结果表明，天然状态的组织培养技术对头颈部肿瘤化学敏感性测试是可行的。
