Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1926 Aug 31;44(3):397-407. doi: 10.1084/jem.44.3.397.
The ultrafilterable constituents of embryonic tissue extract are unable to support cell life in vitro. They stimulate cell migration and possibly multiplication, without increasing the mass of the tissue. Embryonic tissue extract freed from amino acids by dialysis still retains a considerable part of its growth-promoting properties. The area of growth of tissues in embryonic tissue extract free from amino acids is appreciably less than that with the whole extract, probably owing to the denaturation of part of the protein, or perhaps to the inactivation or loss of an enzyme. The addition of either the ultrafilterable components or an artificial mixture of amino acids to this dialyzed extract increases the area of cell migration but does not restore all the activity lost on dialysis. The observed differences in growth of tissue, due to the addition or removal of dialyzable and ultrafilterable constituents of the extract, prove that the amino acids produce a more active cell migration and possibly multiplication, but no building up of new protoplasm.
胚胎组织提取物的可超滤成分在体外无法支持细胞存活。它们刺激细胞迁移并可能增殖，而不会增加组织的质量。通过透析从氨基酸中去除的胚胎组织提取物仍然保留了相当一部分促进生长的特性。从氨基酸中去除的胚胎组织提取物的组织生长面积明显小于整个提取物，这可能是由于部分蛋白质变性，或者可能是由于酶失活或丢失。将可超滤成分或人工混合的氨基酸添加到这种透析提取物中会增加细胞迁移的面积，但不会恢复透析过程中失去的全部活性。由于添加或去除提取物的可透析和可超滤成分而导致的组织生长差异证明，氨基酸会产生更活跃的细胞迁移和可能的增殖，但不会形成新的细胞质。
