Laboratories of The Rockefeller Institute for Medical Research.
J Exp Med. 1928 Apr 30;47(5):821-7. doi: 10.1084/jem.47.5.821.
The experiments reported were designed to determine the influence of malarial infection (Plasmodium inui?), splenectomy, or both combined, upon the course and character of experimental infection with Bartonella bacilliformis in monkeys (Macacus rhesus and M. cynomolgus). Blood withdrawn from a monkey showing spontaneous malarial infection was inoculated intravenously into monkeys (a) 1 month prior to inoculation with virulent verruga material, (b) simultaneously with the verruga material, and (c) during convalescence from verruga infection of moderate severity. All the monkeys contracted the malarial infection and suffered one to three paroxysms during a period of about a month. The verruga lesions appeared in the inoculated animals in due course, were of average size, remained for the usual length of time, and Bartonella bacilliformis was recovered in culture from blood which also contained the plasmodia. The lesions in the convalescent animals continued to heal at the normal rate, and blood cultures were negative for Bartonella bacilliformis, as is usual during convalescence. One of the recovering animals was reinoculated with virulent verruga material a month after the injection of the malarial blood, but neither did new lesions arise nor old ones recur. The malarial infection, therefore, had no effect upon the course of verruga or upon the establishment of immunity to Pattonella bacilliformis, hence it would appear that malaria and verruga may coexist in the same individual without unfavorable effect of one disease upon the course of the other. Similarly, splenectomy led to no appreciable aggravation of Bartonella infection. One monkey subjected to splenectomy and inoculated with verruga material shortly afterwards had an unusually severe reaction, but another, which was infected with material from the first and simultaneously splenectomized, reacted only moderately, while the non-splenectomized control showed a severer type of cutaneous infection. Even the combination of splenectomy and malarial infection did not appreciably aggravate the experimental verruga. Neither relapse of verruga nor reinfection with Bartonella bacilliformis was induced in convalescent or recovered monkeys as a result of splenectomy.
本研究旨在探究疟原虫感染（伊蚊疟原虫）、脾切除术以及二者联合对感染布氏疏螺旋体后猴（恒河猴和食蟹猴）的病程和特征的影响。从一只患有自发性疟原虫感染的猴身上抽取血液，静脉内接种至其他猴（a）在接种强毒野毒株前 1 个月，（b）与野毒株同时，以及（c）野毒株感染中等严重程度的恢复期。所有猴都感染了疟原虫，约一个月内经历了 1-3 次发作。在接种的动物中，适时出现了恰加斯病样疹，大小平均，持续时间正常，从含有疟原虫的血液中也恢复了布氏疏螺旋体的培养。在恢复期动物中，病灶继续正常愈合，且血液培养为布氏疏螺旋体阴性，这在恢复期是常见的。在疟原虫血液注射后 1 个月，一只恢复的动物被重新接种了强毒野毒株，但未出现新的病灶，旧病灶也未复发。因此，疟原虫感染对恰加斯病样疹的病程或对布氏疏螺旋体的免疫建立没有影响，这表明疟疾和恰加斯病样疹可能在同一个体中共存，而不会对另一种疾病的病程产生不利影响。同样，脾切除术也不会导致布氏疏螺旋体感染的明显加重。一只接受脾切除术并随后接种恰加斯病样疹的猴出现了异常严重的反应，但另一只同时感染了第一例并接受脾切除术的猴仅反应适度，而非脾切除术对照则表现出更严重的皮肤感染类型。即使脾切除术和疟原虫感染联合也不会明显加重实验性恰加斯病样疹。脾切除术不会导致恢复期或恢复猴的恰加斯病样疹复发或再次感染布氏疏螺旋体。
