Biological Division, Department of Medicine, Johns Hopkins Medical School and Hospital, Baltimore.
J Exp Med. 1937 Jan 1;65(1):163-76. doi: 10.1084/jem.65.1.163.
Although sera derived from patients at the time of acute, active infection were found to be capable of destroying hemolytic streptococci under aerobic conditions, the organisms retained viability when the tests were performed in the environment of anaerobiosis afforded by a vaseline seal or an anaerobic jar. Within the limitations of the experimental procedures which were employed, the aerobic or anaerobic effect was found to be a reversible reaction. Heating sera at 60 degrees C. for 1 hour inactivated the streptococcidal element in most instances, but not in every case; heating at 56 degrees C. for 1 hour impaired the killing power of half of the specimens which were tested. Sera retained the capacity to destroy hemolytic streptococci when kept in the ice box for 3 weeks; a slight diminution in killing power was noted after 4 weeks. By the methods which were employed, the streptococcidal property of sera could not be correlated with either true antibacterial agglutination or with pseudo-agglutination. The significance of the findings as a basis for analyzing the mechanism of the streptococcidal phenomenon is discussed.
虽然在急性、活跃感染期从患者体内获得的血清在有氧条件下能够破坏溶血性链球菌，但当在凡士林密封或厌氧罐提供的无氧环境中进行测试时，这些生物体仍然保持活力。在采用的实验程序的限制范围内，发现有氧或无氧的影响是一种可逆反应。在大多数情况下，将血清在 60°C 加热 1 小时可使杀菌成分失活，但并非在所有情况下均如此；在 56°C 加热 1 小时会削弱一半测试样本的杀菌能力。将血清保存在冰盒中 3 周后仍保持破坏溶血性链球菌的能力；4 周后，杀菌能力略有下降。通过采用的方法，血清的杀菌特性不能与真正的抗菌凝集作用或假凝集作用相关联。讨论了这些发现作为分析杀菌现象机制的基础的意义。
