Department of Biochemistry, Duke University, School of Medicine, Durham, North Carolina.
J Exp Med. 1943 Jul 1;78(1):1-8. doi: 10.1084/jem.78.1.1.
Comparative immunological measurements have been carried out on crystalline horse serum albumin in the native state and after regeneration from 8 M urea solutions. The mean antigenic activity of the regenerated protein has been found to be less than 10 per cent of that of the native, whereas both antigens proved to be immunologically equivalent. The problem of the relation between protein denaturation and immunological activity has been considered and discussed on the basis of known physical and chemical differences between native and denatured protein.
已经对天然状态和从 8M 尿素溶液中再生后的结晶马血清白蛋白进行了比较免疫学测量。发现再生蛋白的平均抗原活性小于天然蛋白的 10%，而两种抗原在免疫学上是等效的。根据天然和变性蛋白之间已知的物理和化学差异，考虑并讨论了蛋白质变性和免疫学活性之间的关系问题。
