大肠杆菌W7“多层”肽聚糖结构的直接证据：中子小角散射研究
Direct proof of a "more-than-single-layered" peptidoglycan architecture of Escherichia coli W7: a neutron small-angle scattering study.
作者信息
Labischinski H, Goodell E W, Goodell A, Hochberg M L
机构信息
Robert Koch Institute of the Federal Health Office, Berlin, Federal Republic of Germany.
出版信息
J Bacteriol. 1991 Jan;173(2):751-6. doi: 10.1128/jb.173.2.751-756.1991.
Abstract
A neutron small-angle scattering study was performed to determine the thickness and the scattering density profile of isolated peptidoglycan sacculi of Escherichia coli W7 in aqueous suspension (D2O). The maximum thickness (7 +/- 0.5 nm) of the sacculus from the exponential-phase cells was large enough to suggest the existence of a more-than-single-layered architecture. The experimental density profile across the thickness of the sacculus did not allow an unambiguous differentiation between a single-layered architecture characterized by completely extended peptide side chains projecting from the sugar strands or, alternatively, a partially triple layered structure. To resolve this ambiguity, sacculi were labeled with deuterated wall peptides. Comparison of the two experimental profiles indicated that the sacculus is more than single layered across its surface, with about 75 to 80% of its surface single layered and 20 to 25% triple layered.
摘要
进行了一项中子小角散射研究，以确定大肠杆菌W7在水悬浮液（D2O）中分离的肽聚糖囊泡的厚度和散射密度分布。指数期细胞的囊泡最大厚度（7±0.5纳米）足够大，表明存在不止单层的结构。穿过囊泡厚度的实验密度分布不允许在由从糖链伸出的完全伸展的肽侧链表征的单层结构或部分三层结构之间进行明确区分。为了解决这种模糊性，用氘代壁肽标记囊泡。两种实验分布的比较表明，囊泡在其表面上不止单层，其表面约75%至80%为单层，20%至25%为三层。
相似文献
1
Direct proof of a "more-than-single-layered" peptidoglycan architecture of Escherichia coli W7: a neutron small-angle scattering study.大肠杆菌W7“多层”肽聚糖结构的直接证据：中子小角散射研究
J Bacteriol. 1991 Jan;173(2):751-6. doi: 10.1128/jb.173.2.751-756.1991.
2
Molecular organization of Gram-negative peptidoglycan.革兰氏阴性菌肽聚糖的分子结构
Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18953-7. doi: 10.1073/pnas.0808035105. Epub 2008 Nov 24.
3
Elasticity of the sacculus of Escherichia coli.大肠杆菌球囊的弹性
J Bacteriol. 1992 Jul;174(14):4811-9. doi: 10.1128/jb.174.14.4811-4819.1992.
4
Thickness and elasticity of gram-negative murein sacculi measured by atomic force microscopy.通过原子力显微镜测量革兰氏阴性菌胞壁质囊的厚度和弹性。
J Bacteriol. 1999 Nov;181(22):6865-75. doi: 10.1128/JB.181.22.6865-6875.1999.
5
Peptidoglycan structure and architecture.肽聚糖的结构与架构。
FEMS Microbiol Rev. 2008 Mar;32(2):149-67. doi: 10.1111/j.1574-6976.2007.00094.x. Epub 2008 Jan 8.
6
Multilayered distribution of peptidoglycan in the periplasmic space of Escherichia coli.肽聚糖在大肠杆菌周质空间中的多层分布。
J Gen Microbiol. 1989 May;135(5):1243-54. doi: 10.1099/00221287-135-5-1243.
7
Murein (peptidoglycan) structure, architecture and biosynthesis in Escherichia coli.大肠杆菌中胞壁质（肽聚糖）的结构、架构及生物合成
Biochim Biophys Acta. 2008 Sep;1778(9):1714-34. doi: 10.1016/j.bbamem.2007.06.007. Epub 2007 Jun 16.
8
Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli.大肠杆菌承受压力和维持形状的胞壁质囊的生长
Microbiol Mol Biol Rev. 1998 Mar;62(1):181-203. doi: 10.1128/MMBR.62.1.181-203.1998.
9
Soluble nascent peptidoglycan in growing Escherichia coli cells.生长中的大肠杆菌细胞中的可溶性新生肽聚糖。
J Biol Chem. 1980 Oct 25;255(20):9884-90.
10
Contrast variation study of specifically deuterated Escherichia coli ribosomal subunits.特定氘代大肠杆菌核糖体亚基的对比变化研究。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5547-50. doi: 10.1073/pnas.74.12.5547.
引用本文的文献
1
Stress-mediated growth determines division site morphogenesis.应激介导的生长决定分裂位点形态发生。
Proc Natl Acad Sci U S A. 2025 Jul 15;122(28):e2424441122. doi: 10.1073/pnas.2424441122. Epub 2025 Jul 9.
2
Whole-cell modeling of E. coli colonies enables quantification of single-cell heterogeneity in antibiotic responses.大肠杆菌群体的全细胞建模可实现抗生素反应中单细胞异质性的定量分析。
PLoS Comput Biol. 2023 Jun 16;19(6):e1011232. doi: 10.1371/journal.pcbi.1011232. eCollection 2023 Jun.
3
An Updated Model of the Divisome: Regulation of the Septal Peptidoglycan Synthesis Machinery by the Divisome.一个更新的分隔体模型：分隔体对隔膜肽聚糖合成机械的调控。
Int J Mol Sci. 2022 Mar 24;23(7):3537. doi: 10.3390/ijms23073537.
4
Evolution of the analytical scattering model of live .活体分析散射模型的演变
J Appl Crystallogr. 2021 Mar 3;54(Pt 2):473-485. doi: 10.1107/S1600576721000169. eCollection 2021 Apr 1.
5
Peptidoglycan: Structure, Synthesis, and Regulation.肽聚糖：结构、合成与调控。
EcoSal Plus. 2021 Jan;9(2). doi: 10.1128/ecosalplus.ESP-0010-2020.
6
Minimal exposure of lipid II cycle intermediates triggers cell wall antibiotic resistance.脂质 II 循环中间产物的最小暴露会引发细胞壁抗生素耐药性。
Nat Commun. 2019 Jun 21;10(1):2733. doi: 10.1038/s41467-019-10673-4.
7
Cell-Wall Recycling of the Gram-Negative Bacteria and the Nexus to Antibiotic Resistance.革兰氏阴性菌的细胞壁循环与抗生素耐药性的关联。
Chem Rev. 2018 Jun 27;118(12):5952-5984. doi: 10.1021/acs.chemrev.8b00277. Epub 2018 May 30.
8
Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology.聚糖链的分子成像将细胞壁多糖结构与细菌细胞形态联系起来。
Nat Commun. 2018 Mar 28;9(1):1263. doi: 10.1038/s41467-018-03551-y.
9
Structural constraints and dynamics of bacterial cell wall architecture.细菌细胞壁结构的结构限制与动力学
Front Microbiol. 2015 May 8;6:449. doi: 10.3389/fmicb.2015.00449. eCollection 2015.
10
Structure-activity exploration of a small-molecule Lipid II inhibitor.一种小分子脂磷壁酸前体抑制剂的构效关系探索
Drug Des Devel Ther. 2015 Apr 24;9:2383-94. doi: 10.2147/DDDT.S79504. eCollection 2015.
本文引用的文献
1
THE LOCATION OF THE MUCOPEPTIDE IN SECTIONS OF THE CELL WALL OF ESCHERICHIA COLI AND OTHER GRAM-NEGATIVE BACTERIA.黏肽在大肠杆菌及其他革兰氏阴性菌细胞壁切片中的定位。
Can J Microbiol. 1965 Jun;11:547-60. doi: 10.1139/m65-072.
2
The structure of the cell wall of Staphylococcus aureus studied with neutron scattering and magnetic birefringence.用中子散射和磁双折射研究金黄色葡萄球菌细胞壁的结构。
FEBS Lett. 1982 Oct 18;147(2):201-6. doi: 10.1016/0014-5793(82)81042-9.
3
Evidence for involvement of penicillin-binding protein 3 in murein synthesis during septation but not during cell elongation.有证据表明青霉素结合蛋白3参与隔膜形成过程中的胞壁质合成，但不参与细胞伸长过程中的胞壁质合成。
J Bacteriol. 1981 Jan;145(1):333-40. doi: 10.1128/jb.145.1.333-340.1981.
4
The variable T model for gram-negative morphology.革兰氏阴性形态的可变T模型。
J Gen Microbiol. 1984 Sep;130(9):2325-38. doi: 10.1099/00221287-130-9-2325.
5
Molecular model for elongation of the murein sacculus of Escherichia coli.大肠杆菌胞壁质囊伸长的分子模型。
Proc Natl Acad Sci U S A. 1984 Mar;81(6):1844-8. doi: 10.1073/pnas.81.6.1844.
6
Periplasmic gel: new concept resulting from the reinvestigation of bacterial cell envelope ultrastructure by new methods.周质凝胶：通过新方法对细菌细胞壁超微结构进行重新研究得出的新概念。
J Bacteriol. 1984 Oct;160(1):143-52. doi: 10.1128/jb.160.1.143-152.1984.
7
Cellular responses of Bacillus subtilis and Escherichia coli to the Gram stain.枯草芽孢杆菌和大肠杆菌对革兰氏染色的细胞反应。
J Bacteriol. 1983 Nov;156(2):846-58. doi: 10.1128/jb.156.2.846-858.1983.
8
Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers.大肠杆菌细胞壁的超微结构及其组成层的化学性质。
J Ultrastruct Res. 1967 Jul;19(1):45-83. doi: 10.1016/s0022-5320(67)80059-5.
9
Autolytic enzymes and cell division of Escherichia coli.大肠杆菌的自溶酶与细胞分裂
J Mol Biol. 1969 May 14;41(3):419-29. doi: 10.1016/0022-2836(69)90285-x.
10
DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r.快速生长的大肠杆菌B/r分裂周期中的DNA合成
J Mol Biol. 1968 Feb 14;31(3):507-18. doi: 10.1016/0022-2836(68)90424-5.
Zotero 插件