Krishnan M, Burgner J W, Chilton W S, Gelvin S B
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
J Bacteriol. 1991 Jan;173(2):903-5. doi: 10.1128/jb.173.2.903-905.1991.
We have examined the uptake of [14C]octopine and [14C]nopaline by Agrobacterium tumefaciens strains containing the C58 chromosomal background in medium suitable for the induction of vir genes. All strains tested could transport both of these opines, regardless of the presence or type of Ti plasmid (octopine or nopaline) present in the bacterium. The transport of these opines required active cellular metabolism. Nonradioactive octopine, nopaline, and arginine competed effectively with [14C]octopine and [14C]nopaline for transport into A. tumefaciens A136, suggesting that the transport of these opines occurs via an arginine transport pathway not encoded by the Ti plasmid.
我们检测了含有C58染色体背景的根癌土壤杆菌菌株在适合诱导vir基因的培养基中对[14C]章鱼碱和[14C]胭脂碱的摄取情况。所有测试菌株都能转运这两种冠瘿碱，无论细菌中存在的Ti质粒（章鱼碱型或胭脂碱型）情况如何。这些冠瘿碱的转运需要活跃的细胞代谢。非放射性的章鱼碱、胭脂碱和精氨酸能有效地与[14C]章鱼碱和[14C]胭脂碱竞争，从而转运进入根癌土壤杆菌A136，这表明这些冠瘿碱的转运是通过Ti质粒未编码的精氨酸转运途径进行的。
