蛋白质的凝固及其逆转：不溶性球蛋白、可溶性球蛋白和血红素的制备。
PROTEIN COAGULATION AND ITS REVERSAL : THE PREPARATION OF INSOLUBLE GLOBIN, SOLUBLE GLOBIN AND HEME.
机构信息
Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J., and the Hospital of The Rockefeller Institute for Medical Research, New York.
出版信息
J Gen Physiol. 1930 Mar 20;13(4):469-76. doi: 10.1085/jgp.13.4.469.
Abstract
- By a procedure involving the use of acid acetone hemoglobin may be rapidly separated into a precipitate of denatured globin and an acetone solution of heme. 2. By neutralization procedures the denatured globin may be largely converted into a soluble, apparently native form which can combine with heme to form hemoglobin. 3. The heme may be obtained in acetone-free, slightly alkaline solution without the use of strong alkali which may modify the heme.
摘要
通过使用酸丙酮的方法，血红蛋白可以迅速分离成变性球蛋白的沉淀和血红素的丙酮溶液。
通过中和方法，变性球蛋白可以大部分转化为可溶的、明显的天然形式，它可以与血红素结合形成血红蛋白。
血红素可以在没有使用强碱的情况下，从无丙酮、弱碱性溶液中获得，强碱可能会修饰血红素。
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