Department of Pathology, College of Physicians and Surgeons, Columbia University, New York.
J Gen Physiol. 1937 Mar 20;20(4):621-9. doi: 10.1085/jgp.20.4.621.
Digestion of adult tissue with trypsin has been shown to stimulate its initial growth in vitro. This stimulation appeared to result from the removal of an inhibitory material from the tissue due to the proteolytic action of the trypsin. This paper shows that the fluid after the digestion contains material which inhibits the initial growth of adult chicken aorta tissue. This tissue inhibitor has been obtained from chicken, dog, and sheep aortas. It is partly precipitated by an equal volume of alcohol, and is more completely precipitated by the further addition of CaCl(2) (plus a little NaOH). The inhibitor is destroyed at 100 degrees C. but usually withstands 58 degrees C. for 20 minutes. Moderate tryptic digestion renders it soluble without destroying it. It sometimes withstands dialysis, but at other times is lost. Its physical and chemical properties, as far as we know them, coincide with those of lactenin (a bacteriostatic substance in milk). Lactenin was found to inhibit adult tissue growth; but the tissue inhibitor failed to restrain bacterial growth. This tissue inhibitor is believed to play a rôle in limiting the growth of tissue in the adult animal.
用胰蛋白酶消化成年组织已被证明能刺激其在体外的最初生长。这种刺激似乎是由于胰蛋白酶的蛋白水解作用从组织中去除了一种抑制物质。本文表明，消化后的液体含有抑制成年鸡主动脉组织最初生长的物质。这种组织抑制剂已从鸡、狗和羊的主动脉中获得。它部分被等体积的酒精沉淀，进一步用 CaCl(2)（加少量 NaOH）沉淀更完全。抑制剂在 100°C 下被破坏，但通常在 58°C 下耐受 20 分钟。适度的胰蛋白酶消化使其溶解而不破坏它。它有时能耐受透析，但有时会丢失。就我们所知，它的物理和化学性质与乳凝素（牛奶中的一种抑菌物质）相符。乳凝素被发现能抑制成年组织的生长；但组织抑制剂未能抑制细菌的生长。这种组织抑制剂被认为在限制成年动物组织的生长中起作用。
