在溶液中的视紫红质。

ON RHODOPSIN IN SOLUTION.

机构信息

Biological Laboratories of Harvard University, Cambridge.

出版信息

J Gen Physiol. 1938 Jul 20;21(6):795-832. doi: 10.1085/jgp.21.6.795.

DOI:10.1085/jgp.21.6.795

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2141975/

Abstract

The properties of rhodopsin in solution have been examined in preparations from marine fishes, frogs, and mammals. 2. The bleaching of neutral rhodopsin in solution includes a photic and at least three thermal ("dark") processes. Thermal reactions account for approximately half the total fall in extinction at 500 mmicro. 3. Bleaching has been investigated at various pH's from 3.9 to about 11. With increase in pH the velocity of the thermal components increases rapidly. Though the spectrum of rhodopsin itself is scarcely affected by change in pH, the spectra of all product-mixtures following irradiation are highly pH-labile. 4. The spectrum of pure rhodopsin-or of the rhodopsin chromophore-is fixed within narrow limits. The extinction at 400 mmicro lies between 0.20 to 0.32 of that at the maximum. 5. Within the limitations of available data, the spectrum of pure rhodopsin corresponds in form and position with the spectral sensitivity of human rod vision, computed at the retinal surface. 6. The nature of bleaching of rhodopsin in solution, its kinetics, and its significance in the retinal cycle are discussed.

摘要

已经在来自海洋鱼类、青蛙和哺乳动物的制剂中检查了溶液中视紫红质的性质。
中性视紫红质在溶液中的漂白包括光和至少三个热（“暗”）过程。热反应约占 500nm 处消光值总下降的一半。
在 3.9 到约 11 的各种 pH 值下研究了漂白。随着 pH 值的增加，热组分的速度迅速增加。虽然视紫红质本身的光谱几乎不受 pH 值变化的影响，但照射后所有产物混合物的光谱都高度不稳定。
纯视紫红质或视紫红质生色团的光谱在狭窄的范围内固定。在 400nmicro 处的消光值介于最大值的 0.20 到 0.32 之间。
在可用数据的限制内，纯视紫红质的光谱在形式和位置上与在视网膜表面计算的人棒状视敏度的光谱灵敏度相对应。
讨论了溶液中视紫红质漂白的性质、动力学及其在视网膜循环中的意义。