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1
Characterization of human enzymes specific for damaged DNA: resolution of endonuclease for irradiated DNA from an apparent N-glycosidase active on alkylated DNA.对损伤DNA具有特异性的人类酶的特性：从一种对烷基化DNA具有活性的明显N-糖苷酶中解析出辐照DNA的内切核酸酶。
Nucleic Acids Res. 1977 Jul;4(7):2445-54. doi: 10.1093/nar/4.7.2445.
2
Purification and characterization of an endonuclease from Micrococcus luteus that acts on depurinated and carcinogen-modified DNA.从藤黄微球菌中纯化和鉴定一种作用于脱嘌呤和致癌物修饰DNA的核酸内切酶。
Eur J Biochem. 1978 Sep 1;89(2):607-18. doi: 10.1111/j.1432-1033.1978.tb12565.x.
3
Substrate specificity of the ultraviolet-endonuclease from Micrococcus luteus. Endonucleolytic cleavage of depurinated DNA.藤黄微球菌紫外线内切核酸酶的底物特异性。脱嘌呤DNA的内切核酸酶切割
Eur J Biochem. 1976 Oct 1;69(1):265-72. doi: 10.1111/j.1432-1033.1976.tb10882.x.
4
Purification and characterization of human endonucleases specific for damaged DNA. Analysis of lesions induced by ultraviolet or x-radiation.人源特异性损伤DNA内切酶的纯化与特性分析。紫外线或X射线诱导损伤的分析。
Biochim Biophys Acta. 1976 Nov 12;454(1):172-83. doi: 10.1016/0005-2787(76)90363-4.
5
A new endonuclease from Escherichia coli acting at apurinic sites in DNA.一种来自大肠杆菌的新型核酸内切酶，作用于DNA中的脱嘌呤位点。
J Biol Chem. 1977 May 10;252(9):2808-14.
6
Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA.嘧啶二聚体DNA糖基化酶与脱嘌呤/脱嘧啶DNA内切核酸酶的物理关联对紫外线损伤DNA的修复至关重要。
Proc Natl Acad Sci U S A. 1981 May;78(5):2742-6. doi: 10.1073/pnas.78.5.2742.
7
Partial purification of endonuclease activity from human lymphoblasts. Separation of activities for depurinated DNA and DNA irradiated with ultraviolet light.从人淋巴母细胞中部分纯化核酸内切酶活性。对脱嘌呤DNA和紫外线照射的DNA的活性进行分离。
Biochim Biophys Acta. 1975 Oct 1;407(2):191-9. doi: 10.1016/0005-2787(75)90284-1.
8
Apurinic endonuclease from Saccharomyces cerevisiae.来自酿酒酵母的脱嘌呤内切酶。
Biochem J. 1981 May 1;195(2):407-17. doi: 10.1042/bj1950407.
9
Action of bacteriophage T4 ultraviolet endonuclease on duplex DNA containing one ultraviolet-irradiated strand.噬菌体T4紫外线内切核酸酶对含有一条紫外线照射链的双链DNA的作用。
J Biol Chem. 1975 Nov 25;250(22):8748-52.
10
Repair of DNA damaged by methyl methanesulfonate in bacteriophage T4.噬菌体T4中被甲磺酸甲酯损伤的DNA修复
Biochim Biophys Acta. 1976 Aug 18;442(2):208-15. doi: 10.1016/0005-2787(76)90491-3.

引用本文的文献

1
Hydroxymethyluracil DNA glycosylase in mammalian cells.哺乳动物细胞中的羟甲基尿嘧啶DNA糖基化酶
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4003-7. doi: 10.1073/pnas.81.13.4003.
2
UV-endonuclease from calf thymus with specificity toward pyrimidine dimers in DNA.来自小牛胸腺的对DNA中嘧啶二聚体具有特异性的紫外线内切核酸酶。
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3746-50. doi: 10.1073/pnas.76.8.3746.

本文引用的文献

1
Properties of bacteriophage PM2: a lipid-containing bacterial virus.噬菌体PM2的特性：一种含脂质的细菌病毒。
Virology. 1968 Apr;34(4):738-47. doi: 10.1016/0042-6822(68)90094-9.
2
T4 endonuclease involved in repair of DNA.参与DNA修复的T4核酸内切酶。
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1839-45. doi: 10.1073/pnas.67.4.1839.
3
DNA of bacteriophage PM2: a closed circular double-stranded molecule.噬菌体PM2的DNA：一种封闭的环状双链分子。
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1164-8. doi: 10.1073/pnas.63.4.1164.
4
Enzymatic repair of DNA. 3. Properties of the UV-endonuclease and UV-exonuclease.DNA的酶促修复。3. 紫外线内切核酸酶和紫外线外切核酸酶的特性。
Biochemistry. 1971 Aug 31;10(18):3315-24. doi: 10.1021/bi00794a001.
5
Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease.噬菌体T4对紫外线照射的脱氧核糖核酸的暗修复：一种二聚体特异性噬菌体诱导的内切核酸酶的纯化与特性分析
J Bacteriol. 1971 May;106(2):500-7. doi: 10.1128/jb.106.2.500-507.1971.
6
A human endonuclease activity for gamma-irradiated DNA.一种针对γ射线辐照DNA的人类核酸内切酶活性。
Biophys J. 1973 Apr;13(4):399-401. doi: 10.1016/S0006-3495(73)85993-4.
7
The enzymatic release of O6-methylguanine and 3-methyladenine from DNA reacted with the carcinogen N-methyl-N-nitrosourea.与致癌物N-甲基-N-亚硝基脲反应的DNA中O6-甲基鸟嘌呤和3-甲基腺嘌呤的酶促释放。
Proc Natl Acad Sci U S A. 1974 May;71(5):2022-6. doi: 10.1073/pnas.71.5.2022.
8
Properties of the endonuclease for depurinated DNA from Escherichia coli.来自大肠杆菌的脱嘌呤DNA的核酸内切酶特性。
Can J Biochem. 1972 Nov;50(11):1199-209. doi: 10.1139/o72-163.
9
Endonucleolytic activity from Micrococcus luteus that acts on -ray-induced damage in plasmid DNA of Escherichia coli minicells.藤黄微球菌的核酸内切酶活性作用于大肠杆菌微小细胞质粒DNA中的γ射线诱导损伤。
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2927-31. doi: 10.1073/pnas.69.10.2927.
10
Repair enzyme suggested by mammalian endonuclease activity specific for ultraviolet-irradiated DNA.由对紫外线照射的DNA具有特异性的哺乳动物内切核酸酶活性所提示的修复酶。
Nat New Biol. 1972 Oct 11;239(93):172-3. doi: 10.1038/newbio239172a0.

对损伤DNA具有特异性的人类酶的特性：从一种对烷基化DNA具有活性的明显N-糖苷酶中解析出辐照DNA的内切核酸酶。

Characterization of human enzymes specific for damaged DNA: resolution of endonuclease for irradiated DNA from an apparent N-glycosidase active on alkylated DNA.

作者信息

Brent T P

出版信息

Nucleic Acids Res. 1977 Jul;4(7):2445-54. doi: 10.1093/nar/4.7.2445.

DOI:10.1093/nar/4.7.2445
PMID:198741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342577/
Abstract

An endonuclease partially purified from human lymphoblasts, and active against ultraviolet-irradiated DNA, was found to act additionally on DNA damaged by either x-radiation or methylmethanesulfonate. To determine if these activities were truly endonucleolytic, the reaction products were analyzed under conditions that prevented conversion of apurinic or apyrimidinic sites to single-strand breaks. With either ultraviolet- or x-irradiated DNA, strand breakage remained maximal, hence confirming the endonucleolytic character of the enzyme. By contrast, with DNA alkylated with methylmethanesulfonate, strand breakage was sharply reduced. Additional experiments indicated that the activity for alkylated DNA induces strand breaks only in concert with a purified endonuclease specific for apurinic sites, suggesting that it is an N-glycosidase that depurinates alkylated bases. This enzyme was separated from the endonuclease specific for irradiated DNA, by chromatography on DNA-agarose.

摘要

从人淋巴母细胞中部分纯化得到一种核酸内切酶，它对紫外线照射的DNA有活性，还发现它对受X射线或甲磺酸甲酯损伤的DNA也有作用。为了确定这些活性是否真的是核酸内切酶活性，在防止脱嘌呤或脱嘧啶位点转化为单链断裂的条件下分析反应产物。无论是紫外线还是X射线照射的DNA，链断裂都保持在最大值，从而证实了该酶的核酸内切酶特性。相比之下，对于用甲磺酸甲酯烷基化的DNA，链断裂明显减少。进一步的实验表明，烷基化DNA的活性仅与一种对脱嘌呤位点具有特异性的纯化核酸内切酶协同作用时才诱导链断裂，这表明它是一种使烷基化碱基脱嘌呤的N-糖苷酶。通过在DNA-琼脂糖上进行层析，将这种酶与对辐照DNA具有特异性的核酸内切酶分离。