Hwa Hsiao-Lin, Ko Tsang-Ming, Chen Yao-Chang, Chang Yih-Yuan, Tseng Li-Hui, Su Yi-Ning, Lee James Chun-I
Department and Graduate Institute of Forensic Medicine, College of Medicine, National Taiwan University Hospital and National Taiwan University, No. 7, Chung-Shan South Road, Taipei 100, Taiwan.
J Forensic Sci. 2010 Jan;55(1):167-70. doi: 10.1111/j.1556-4029.2009.01195.x. Epub 2009 Oct 23.
The cytochrome b gene (MTCYB) has been widely used in taxonomic research. In this study, the sequence polymorphism of the MTCYB gene was determined in 417 subjects of eight populations living in Taiwan (Taiwanese Han, indigenous Taiwanese, Tao, mainland Chinese, Filipino, Thai, Vietnamese, and Caucasian). Sequence variation from the revised Cambridge Reference Sequence and genetic distance between these populations were analyzed. There were 108 variable positions with a total of 99 haplotypes. Population-specific positions of MTCYB gene were noted in Tao and Caucasian populations. There were statistically significant differences of genetic distance between Taiwanese Han and Caucasian, between Taiwanese Han and Tao, and between Taiwanese Han and Filipino. A phylogenetic tree presents the genetic distances between these populations. In conclusion, there are sufficient sequence polymorphisms of the MTCYB gene in individuals of different populations, which may be used in the analyses of human ethnic groups in forensic casework.
细胞色素b基因（MTCYB）已广泛应用于分类学研究。在本研究中，测定了居住在台湾的八个群体（台湾汉族、台湾原住民、达悟族、中国大陆人、菲律宾人、泰国人、越南人和高加索人）417名受试者的MTCYB基因序列多态性。分析了与修订的剑桥参考序列的序列变异以及这些群体之间的遗传距离。共有108个可变位点，总计99种单倍型。在达悟族和高加索人群体中发现了MTCYB基因的群体特异性位点。台湾汉族与高加索人、台湾汉族与达悟族、台湾汉族与菲律宾人之间的遗传距离存在统计学显著差异。系统发育树展示了这些群体之间的遗传距离。总之，不同群体个体的MTCYB基因存在足够的序列多态性，可用于法医案件工作中的人类族群分析。
