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蛙卵母细胞在显微注射禽成髓细胞瘤病毒RNA后，能合成并完全加工病毒粒子结构蛋白的前体多肽。

Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA.

作者信息

Ghysdael J, Hubert E, Trávnícek M, Bolognesi D P, Burny A, Cleuter Y, Huez G, Kettmann R, Marbaix G, Portetelle D, Chantrenne H

出版信息

Proc Natl Acad Sci U S A. 1977 Aug;74(8):3230-4. doi: 10.1073/pnas.74.8.3230.

DOI:10.1073/pnas.74.8.3230
PMID:198776
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC431509/
Abstract

After microinjection of Xenopus laevis oocytes with RNA from avian myeloblastosis virus, viral structural proteins p27, p19, p15, and p12 are formed by a sequence of posttranslational cleavages of a high-molecular-weight precursor polypeptide. The 60-70S RNA aggregate or its 30-40S RNA subunits obtained by heat or formamide treatment possess the same ability to serve as template in X. laevis oocytes. The processing pattern of virus-specific precursor polypeptides is the same in X. laevis oocytes as in chick embryo fibroblasts infected with avian myeloblastosis virus, but the processing takes place at a much slower rate.

摘要

用禽成髓细胞瘤病毒的RNA显微注射非洲爪蟾卵母细胞后，病毒结构蛋白p27、p19、p15和p12是由一种高分子量前体多肽经一系列翻译后切割形成的。通过加热或甲酰胺处理获得的60 - 70S RNA聚集体或其30 - 40S RNA亚基在非洲爪蟾卵母细胞中具有相同的作为模板的能力。病毒特异性前体多肽的加工模式在非洲爪蟾卵母细胞中与感染禽成髓细胞瘤病毒的鸡胚成纤维细胞中相同，但加工速度要慢得多。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/431509/ec058ec15e66/pnas00030-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/431509/f97b3d739cf8/pnas00030-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/431509/dffba65640cd/pnas00030-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/431509/b525fc19b478/pnas00030-0134-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/431509/ec058ec15e66/pnas00030-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/431509/f97b3d739cf8/pnas00030-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/431509/dffba65640cd/pnas00030-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/431509/b525fc19b478/pnas00030-0134-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f03/431509/ec058ec15e66/pnas00030-0135-a.jpg

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1
Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA.蛙卵母细胞在显微注射禽成髓细胞瘤病毒RNA后，能合成并完全加工病毒粒子结构蛋白的前体多肽。
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3230-4. doi: 10.1073/pnas.74.8.3230.
2
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Translation of 35S RNA from avian myeloblastosis virus into viral specific polypeptides in Xenopus oocytes.禽成髓细胞瘤病毒35S RNA在非洲爪蟾卵母细胞中翻译为病毒特异性多肽。
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A cell-free mammalian protein-synthesizing system stimulated by RNA from avian myeloblastosis virus.一种由禽成髓细胞瘤病毒RNA刺激的无细胞哺乳动物蛋白质合成系统。
Biochim Biophys Acta. 1975 Jul 23;395(4):509-24. doi: 10.1016/0005-2787(75)90074-x.

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本文引用的文献

1
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
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Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.利用蛙卵和卵母细胞研究信使核糖核酸及其在活细胞中的翻译。
Nature. 1971 Sep 17;233(5316):177-82. doi: 10.1038/233177a0.
3
Calf crystallin synthesis in frog cells: the translation of lens-cell 14S RNA in oocytes.蛙细胞中牛晶体蛋白的合成：卵母细胞中晶状体细胞14S RNA的翻译。
禽肉瘤病毒gag前体多肽在哺乳动物细胞中不被加工。
J Virol. 1982 Nov;44(2):725-30. doi: 10.1128/JVI.44.2.725-730.1982.
4
Coexpression of N-methyl-D-aspartate and phencyclidine receptors in Xenopus oocytes injected with rat brain mRNA.注射大鼠脑信使核糖核酸的非洲爪蟾卵母细胞中 N-甲基-D-天冬氨酸受体和苯环利定受体的共表达
Proc Natl Acad Sci U S A. 1988 May;85(9):3250-4. doi: 10.1073/pnas.85.9.3250.
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Post-synthetic fate of the translation products of messenger RNA microinjected into Xenopus oocytes.显微注射到非洲爪蟾卵母细胞中的信使核糖核酸翻译产物的合成后命运。
Mol Biol Rep. 1979 Dec 31;5(4):199-208. doi: 10.1007/BF00782889.
6
Biochemical properties of p15-associated protease in an avian RNA tumor virus.禽RNA肿瘤病毒中与p15相关蛋白酶的生化特性
J Virol. 1978 Oct;28(1):106-18. doi: 10.1128/JVI.28.1.106-118.1978.
7
Immunological purification and partial characterization of variant-specific surface antigen messenger RNA of Trypanosoma brucei brucei.布氏布氏锥虫变异特异性表面抗原信使核糖核酸的免疫纯化及部分特性分析
Nucleic Acids Res. 1979 Oct 10;7(3):595-609. doi: 10.1093/nar/7.3.595.
8
Translation of bovine leukemia virus virion RNAs in heterologous protein-synthesizing systems.牛白血病病毒病毒粒子RNA在异源蛋白质合成系统中的翻译。
J Virol. 1979 Mar;29(3):1087-98. doi: 10.1128/JVI.29.3.1087-1098.1979.
9
Virus-coded origin of a 32,000-dalton protein from avian retrovirus cores: structural relatedness of p32 and the beta polypeptide of the avian retrovirus DNA polymerase.禽逆转录病毒核心中一种32,000道尔顿蛋白质的病毒编码起源：p32与禽逆转录病毒DNA聚合酶β多肽的结构相关性
J Virol. 1978 Oct;28(1):279-91. doi: 10.1128/JVI.28.1.279-291.1978.
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4
Subunits of oncornavirus high-molecular-weight RNA. I. Stepwise conversion of 60 S AMV (avian myeloblastosis virus) RNA to subunits.肿瘤病毒高分子量RNA的亚基。I. 禽成髓细胞瘤病毒（AMV）60S RNA逐步转化为亚基。
Biochem Biophys Res Commun. 1973 Jul 2;53(1):217-23. doi: 10.1016/0006-291x(73)91422-8.
5
Identification of a large polypeptide precursor of avian oncornavirus proteins.禽肿瘤病毒蛋白大多肽前体的鉴定
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1734-8. doi: 10.1073/pnas.70.6.1734.
6
Protein synthesis directed by encephalomyocarditis virus mRNA. 3. Discrete polypeptides translated from a monocistronic messenger in vitro.由脑心肌炎病毒信使核糖核酸指导的蛋白质合成。3. 从单顺反子信使核糖核酸体外翻译出的离散多肽。
Arch Biochem Biophys. 1972 Dec;153(2):706-13. doi: 10.1016/0003-9861(72)90389-x.
7
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Proc Natl Acad Sci U S A. 1972 Dec;69(12):3665-9. doi: 10.1073/pnas.69.12.3665.
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J Virol. 1971 Nov;8(5):730-41. doi: 10.1128/JVI.8.5.730-741.1971.
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A proposed nomenclature for the virion proteins of oncogenic RNA viruses.一种关于致癌RNA病毒病毒粒子蛋白的拟用命名法。
Virology. 1974 Aug;60(2):595-600. doi: 10.1016/0042-6822(74)90356-0.
10
Translation of murine leukemia virus RNA in cell-free systems from animal cells.小鼠白血病病毒RNA在动物细胞无细胞体系中的翻译。
J Virol. 1976 May;18(2):627-35. doi: 10.1128/JVI.18.2.627-635.1976.