Ascione R, Smida J, Robey W G, Vande Woude G F
Biochim Biophys Acta. 1975 Jul 23;395(4):509-24. doi: 10.1016/0005-2787(75)90074-x.
Carcinoma cells, oncornavirus-infected cells and fetal bovine tissue provide salt wash ribosomal factors capable of responding to avian myeloblastosis virus (AM virus)-RNA and stimulating the incorporation of amino acids into proteins as well as catalyzing the binding of N-acetylated (35S) methionyl-tRNA. The exogenously dependent amino acid incorporation system is stimulated by the high molecular weight species of AM virus-RNA only, particularly the fraction containing polyadenylate (poly(A)) residues; the system is also markedly inhibited by the low molecular weight AM virus-RNA species. Activity for the exogenous system displays very definite divalent/monovalent cation optima and requires the presence of mammalian transfer RNA.
癌细胞、被肿瘤病毒感染的细胞以及胎牛组织可提供盐洗核糖体因子，这些因子能够对禽成髓细胞瘤病毒（AM病毒）-RNA作出反应，刺激氨基酸掺入蛋白质，并催化N-乙酰化（35S）甲硫氨酰-tRNA的结合。外源性依赖氨基酸掺入系统仅受AM病毒-RNA的高分子量种类刺激，特别是含有聚腺苷酸（poly(A)）残基的部分；该系统也受到AM病毒-RNA低分子量种类的显著抑制。外源性系统的活性表现出非常明确的二价/单价阳离子最佳值，并且需要哺乳动物转移RNA的存在。
