School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu 702-701, Korea.
Molecules. 2010 May 14;15(5):3507-16. doi: 10.3390/molecules15053507.
In this study, we investigated the antifungal activity and mechanism of action of (+)-pinoresinol, a biphenolic compound isolated from the herb Sambucus williamsii,used in traditional medicine. (+)-Pinoresinol displays potent antifungal properties without hemolytic effects on human erythrocytes. To understand the antifungal mechanism of (+)-pinoresinol, we conducted fluorescence experiments on the human pathogen Candida albicans. Fluorescence analysis using 1,6-diphenyl-1,3,5-hexatriene (DPH) indicated that the (+)-pinoresinol caused damage to the fungal plasma membrane. This result was confirmed by using rhodamine-labeled giant unilamellar vesicle (GUV) experiments. Therefore, the present study indicates that (+)-pinoresinol possesses fungicidal activities and therapeutic potential as an antifungal agent for the treatment of fungal infectious diseases in humans.
在这项研究中，我们研究了 (+)-松脂醇，一种从传统药物中提取的双酚化合物，对真菌的抗真菌活性和作用机制。(+)-松脂醇对人类红细胞没有溶血作用，但具有很强的抗真菌作用。为了了解 (+)-松脂醇的抗真菌机制，我们对人类病原体白色念珠菌进行了荧光实验。使用 1,6-二苯基-1,3,5-己三烯 (DPH) 的荧光分析表明，(+)-松脂醇导致真菌质膜损伤。使用罗丹明标记的巨大单层囊泡 (GUV) 实验证实了这一结果。因此，本研究表明，(+)-松脂醇具有杀菌活性和治疗潜力，可作为治疗人类真菌感染疾病的抗真菌药物。
