Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, USA.
Gastrointest Endosc. 2010 Feb;71(2):346-53. doi: 10.1016/j.gie.2009.07.007. Epub 2009 Oct 31.
Random biopsy esophageal surveillance can be subject to sampling errors, resulting in diagnostic uncertainty. Optical frequency domain imaging (OFDI) is a high-speed, 3-dimensional endoscopic microscopy technique. When deployed through a balloon-centering catheter, OFDI can automatically image the entire distal esophagus (6.0 cm length) in approximately 2 minutes.
To test a new platform for guided biopsy that allows the operator to select target regions of interest on an OFDI dataset, and then use a laser to mark the esophagus at corresponding locations. The specific goals include determining the optimal laser parameters, testing the accuracy of the laser marking process, evaluating the endoscopic visibility of the laser marks, and assessing the amount of mucosal damage produced by the laser.
Experimental study conducted in 5 swine in vivo.
Massachusetts General Hospital.
Success rate, including endoscopic visibility of laser marks and accuracy of the laser marking process for selected target sites, and extent of the thermal damage caused by the laser marks.
All of the laser-induced marks were visible by endoscopy. Target locations were correctly marked with a success rate of 97.07% (95% confidence interval, 89.8%-99.7%). Thermal damage was limited to the superficial layers of the mucosa and was observed to partially heal within 2 days.
An animal study with artificially placed targets to simulate pathology.
The study demonstrates that laser marking of esophageal sites identified in comprehensive OFDI datasets is feasible and can be performed with sufficient accuracy, precision, and visibility to guide biopsy in vivo.
随机活检食管监测可能存在采样误差，导致诊断不确定。光学频域成像（OFDI）是一种高速、三维内镜显微镜技术。当通过球囊中心导管部署时，OFDI 可以在大约 2 分钟内自动成像整个远端食管（6.0 厘米长度）。
测试一种新的引导活检平台，该平台允许操作员在 OFDI 数据集上选择感兴趣的目标区域，然后使用激光在相应位置标记食管。具体目标包括确定最佳激光参数、测试激光标记过程的准确性、评估激光标记的内镜可视性以及评估激光产生的粘膜损伤量。
在 5 头猪体内进行的实验研究。
马萨诸塞州综合医院。
成功率，包括激光标记的内镜可见度和所选目标部位激光标记过程的准确性，以及激光标记引起的热损伤程度。
所有激光诱导的标记都可以通过内窥镜看到。目标位置的标记成功率为 97.07%（95%置信区间，89.8%-99.7%）。热损伤仅限于粘膜的浅层，在 2 天内观察到部分愈合。
一种使用人工放置的目标模拟病理学的动物研究。
该研究表明，在全面的 OFDI 数据集中标记食管部位的激光标记是可行的，并且可以以足够的准确性、精度和可视性进行，以指导体内活检。
