Research Institute for Bioresources, Okayama University, Kurashiki 710-0046, Japan.
Plant Cell. 2009 Oct;21(10):3339-49. doi: 10.1105/tpc.109.070771. Epub 2009 Oct 30.
Aluminum (Al) toxicity is the major limiting factor of crop production on acid soils, but some plant species have evolved ways of detoxifying Al. Here, we report a C2H2-type zinc finger transcription factor ART1 (for Al resistance transcription factor 1), which specifically regulates the expression of genes related to Al tolerance in rice (Oryza sativa). ART1 is constitutively expressed in the root, and the expression level is not affected by Al treatment. ART1 is localized in the nucleus of all root cells. A yeast one-hybrid assay showed that ART1 has a transcriptional activation potential and interacts with the promoter region of STAR1, an important factor in rice Al tolerance. Microarray analysis revealed 31 downstream transcripts regulated by ART1, including STAR1 and 2 and a couple of homologs of Al tolerance genes in other plants. Some of these genes were implicated in both internal and external detoxification of Al at different cellular levels. Our findings shed light on comprehensively understanding how plants detoxify aluminum to survive in an acidic environment.
铝（Al）毒性是酸性土壤上作物生产的主要限制因素，但一些植物物种已经进化出了解毒 Al 的方法。在这里，我们报告了一个 C2H2 型锌指转录因子 ART1（用于铝抗性转录因子 1），它特异性地调节水稻（Oryza sativa）中与 Al 耐受性相关的基因的表达。ART1 在根中组成型表达，并且表达水平不受 Al 处理的影响。ART1 定位于所有根细胞的细胞核中。酵母单杂交测定表明 ART1 具有转录激活潜力，并与 STAR1 的启动子区域相互作用，STAR1 是水稻 Al 耐受性的一个重要因素。微阵列分析显示，ART1 调控了 31 个下游转录本，包括 STAR1 和 2 以及其他植物中一些 Al 耐受性基因的同源物。这些基因中的一些参与了不同细胞水平的 Al 的内部和外部解毒。我们的研究结果阐明了植物如何解毒铝以在酸性环境中生存的综合理解。
