Research Institute for Applied Mechanics, Kyushu University, Kasuga-koen 6-1, Kasuga, Fukuoka 816-8580, Japan.
Biomed Mater. 2009 Dec;4(6):065003. doi: 10.1088/1748-6041/4/6/065003.
The surface of commercially pure titanium was modified by anodization treatment in a phosphoric acid solution at different voltages: 100 V, 200 V and 300 V. The surface characteristics of anodic TiO2 layers and their influence on the cell response were investigated. Micrographs by scanning electron microscopy revealed that the dense and uniform oxide layer obtained at 100 V exhibits a nanostructured surface which is similar to the surface of natural tooth cementum. In contrast, porous oxide layers without nanometer features were produced at higher voltages. Thin film x-ray diffraction analysis confirmed the existence of anatase in the oxide layer obtained at 300 V, but not in oxide layers obtained at 100 V and 200 V. The in vitro biocompatibility study of oxide layers demonstrated greater cell adhesion and proliferation of the oxide layer obtained at 100 V compared to the other two kinds of oxide layers.
100V、200V 和 300V。研究了阳极 TiO2 层的表面特性及其对细胞反应的影响。扫描电子显微镜的显微照片显示，在 100V 时获得的致密且均匀的氧化层呈现出纳米结构的表面，类似于天然牙骨质的表面。相比之下，在较高电压下会产生没有纳米特征的多孔氧化层。薄膜 X 射线衍射分析证实，在 300V 时获得的氧化层中存在锐钛矿，但在 100V 和 200V 时获得的氧化层中不存在。氧化层的体外生物相容性研究表明，与其他两种氧化层相比，在 100V 时获得的氧化层具有更大的细胞黏附和增殖。
