Marques Isabella da Silva Vieira, da Cruz Nilson Cristino, Landers Richard, Yuan Judy Chia-Chun, Mesquita Marcelo Ferraz, Sukotjo Cortino, Mathew Mathew T, Barão Valentim Adelino Ricardo
Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil.
Laboratory of Technological Plasmas, Engineering College, Univ Estadual Paulista (UNESP), Av Três de Março, 511, Sorocaba, São Paulo 18087-180, Brazil.
Biointerphases. 2015 Dec 7;10(4):041002. doi: 10.1116/1.4932579.
The objectives of the present study were to produce bioactive coatings in solutions containing Ca, P, and Si by plasma electrolytic oxidation (PEO) on commercially pure titanium, to investigate the influence of different electrolytes concentration and treatment duration on the produced anodic films and to evaluate biocompatibility properties. The anodic films were characterized using scanning electron microscopy, energy-dispersive spectroscopy, atomic force microscopy, and x-ray diffraction and x-ray photoelectron spectroscopies. The surface energy and roughness were also evaluated. PEO process parameters influenced the crystalline structure formation and surface topography of the anodic films. Higher Ca content produced larger porous (volcanolike appearance) and thicker oxide layers when compared to the lower content. Treatment duration did not produce any topography difference. The treatment modified the surface chemistry, producing an enriched oxide layer with bioactive elements in the form of phosphate compounds, which may be responsible for mimicking bone surface. In addition, a rough surface with increased surface energy was generated. Optimal spreading and proliferation of human mesenchymal stem cells was achieved by PEO treatment, demonstrating excellent biocompatibility of the surface. The main finding is that the biofunctionalization with higher Ca/P on Ti-surface can improve surface features, potentially considered as a candidate for dental implants.
本研究的目的是通过在商业纯钛上进行等离子体电解氧化（PEO），在含有钙、磷和硅的溶液中制备生物活性涂层，研究不同电解质浓度和处理时间对所制备阳极膜的影响，并评估生物相容性。使用扫描电子显微镜、能量色散光谱、原子力显微镜、X射线衍射和X射线光电子能谱对阳极膜进行表征。还评估了表面能和粗糙度。PEO工艺参数影响阳极膜的晶体结构形成和表面形貌。与较低含量相比，较高的钙含量产生更大的多孔结构（类似火山外观）和更厚的氧化层。处理时间未产生任何形貌差异。该处理改变了表面化学性质，产生了富含生物活性元素的氧化层，其形式为磷酸盐化合物，这可能是模拟骨表面的原因。此外，还产生了具有增加表面能的粗糙表面。通过PEO处理实现了人间充质干细胞的最佳铺展和增殖，证明了该表面具有优异的生物相容性。主要发现是钛表面上较高的钙/磷生物功能化可以改善表面特性，有可能被视为牙科植入物的候选材料。
