Yan Weihong, Apweiler Rolf, Balgley Brian M, Boontheung Pinmanee, Bundy Jonathan L, Cargile Benjamin J, Cole Steve, Fang Xueping, Gonzalez-Begne Mireya, Griffin Timothy J, Hagen Fred, Hu Shen, Wolinsky Lawrence E, Lee Cheng S, Malamud Daniel, Melvin James E, Menon Rajasree, Mueller Michael, Qiao Renli, Rhodus Nelson L, Sevinsky Joel R, States David, Stephenson James L, Than Shawn, Yates John R, Yu Weixia, Xie Hongwei, Xie Yongming, Omenn Gilbert S, Loo Joseph A, Wong David T
Department of Chemistry and Biochemistry, University of California-Los Angeles, Los Angeles, CA, USA.
Proteomics Clin Appl. 2009 Jan 1;3(1):116-134. doi: 10.1002/prca.200800140.
The proteome of human salivary fluid has the potential to open new doors for disease biomarker discovery. A recent study to comprehensively identify and catalog the human ductal salivary proteome led to the compilation of 1166 proteins. The protein complexity of both saliva and plasma is large, suggesting that a comparison of these two proteomes will provide valuable insight into their physiological significance and an understanding of the unique and overlapping disease diagnostic potential that each fluid provides. To create a more comprehensive catalog of human salivary proteins, we have first compiled an extensive list of proteins from whole saliva (WS) identified through MS experiments. The WS list is thereafter combined with the proteins identified from the ductal parotid, and submandibular and sublingual (parotid/SMSL) salivas. In parallel, a core dataset of the human plasma proteome with 3020 protein identifications was recently released. A total of 1939 nonredundant salivary proteins were compiled from a total of 19 474 unique peptide sequences identified from whole and ductal salivas; 740 out of the total 1939 salivary proteins were identified in both whole and ductal saliva. A total of 597 of the salivary proteins have been observed in plasma. Gene ontology (GO) analysis showed similarities in the distributions of the saliva and plasma proteomes with regard to cellular localization, biological processes, and molecular function, but revealed differences which may be related to the different physiological functions of saliva and plasma. The comprehensive catalog of the salivary proteome and its comparison to the plasma proteome provides insights useful for future study, such as exploration of potential biomarkers for disease diagnostics.
人类唾液蛋白质组有潜力为疾病生物标志物的发现打开新的大门。最近一项全面鉴定和编目人类导管唾液蛋白质组的研究，汇总了1166种蛋白质。唾液和血浆的蛋白质复杂性都很高，这表明对这两种蛋白质组进行比较，将为它们的生理意义提供有价值的见解，并有助于理解每种液体所具有的独特且相互重叠的疾病诊断潜力。为了创建更全面的人类唾液蛋白质目录，我们首先汇总了通过质谱实验从全唾液（WS）中鉴定出的大量蛋白质列表。此后，将该WS列表与从腮腺、颌下腺和舌下腺（腮腺/颌下腺/舌下腺）唾液中鉴定出的蛋白质相结合。与此同时，最近发布了一个包含3020个蛋白质鉴定信息的人类血浆蛋白质组核心数据集。从全唾液和导管唾液中鉴定出的总共19474个独特肽序列中，汇总出了1939种非冗余唾液蛋白质；在全唾液和导管唾液中都鉴定出了1939种唾液蛋白质中的740种。在血浆中观察到了总共597种唾液蛋白质。基因本体（GO）分析表明，唾液和血浆蛋白质组在细胞定位、生物学过程和分子功能方面的分布存在相似性，但也揭示了可能与唾液和血浆不同生理功能相关的差异。唾液蛋白质组的综合目录及其与血浆蛋白质组的比较，为未来的研究提供了有用的见解，例如探索用于疾病诊断的潜在生物标志物。
