Palya V, Belák S, Pálfi V
Zentralbl Veterinarmed B. 1977 Aug;24(7):529-41. doi: 10.1111/j.1439-0450.1977.tb01022.x.
Adenovirus strain GY/14 isolated during a natural outbreak was used in experimental infection. Three weeks old lambs responded with temperature rise, respiratory symptoms and diarrhoea to the infection. Infection spread to a contact animal, too. Reisolation of the virus was successful from the nasal discharge and feces from the 3rd to 10th, and the 3rd to 5th day following infection, respectively. In the killed experimental animals the pathological and histological changes observed were similar to those observed in natural cases. On comparing the natural outbreaks with the experimental infection the only difference appeared in the severity of the changes. Following the experimental infection characteristic nuclear inclusions appeared in the nasal and bronchiolar epithelium, in the alveolar septal cells and in the reticular cells of the lymph nodes. Epizootiologic observations and experimental results confirm the assumption that our adenovirus strains isolated from natural cases are pathogenic for lambs.
在一次自然暴发期间分离出的腺病毒株GY/14被用于实验性感染。三周龄的羔羊感染后出现体温升高、呼吸道症状和腹泻。感染也传播到了一只接触动物身上。分别在感染后的第3至10天以及第3至5天，从鼻分泌物和粪便中成功重新分离出病毒。在处死的实验动物中，观察到的病理和组织学变化与自然病例中观察到的相似。将自然暴发与实验性感染进行比较时，唯一的差异在于变化的严重程度。实验性感染后，在鼻和细支气管上皮、肺泡隔细胞以及淋巴结的网状细胞中出现了特征性核内包涵体。流行病学观察和实验结果证实了以下假设：我们从自然病例中分离出的腺病毒株对羔羊具有致病性。
