Bauer Natali, Eralp Oya, Moritz Andreas
Department of Veterinary Clinical Sciences, Clinical Pathophysiology and Clinical Pathology, Justus-Liebig University Giessen, Giessen, Germany.
J Vet Diagn Invest. 2009 Nov;21(6):803-14. doi: 10.1177/104063870902100606.
Reference intervals for coagulation parameters have been rarely determined in dogs for the STA Compact automated coagulation analyzer, so it is the aim of the current study to validate assays and establish reference ranges for its use in canine specimens. Coagulation parameters were assessed in 56 healthy dogs with a median age of 2 years and evenly distributed sex. The 95% reference intervals were as follows: 1-stage prothrombin time = 5.7-8.0 sec; activated partial thromboplastin time (APTT) = 10.0-14.3 sec; thrombin time (TT) = 11.9-18.3 sec; fibrinogen = 1.3-3.1 g/l; antithrombin (AT) = 107.9-128.0%; D-dimer = 0.023-0.65 microg/ml; anti-factor Xa = 0.04-0.26 IU/l; and activated protein C (APC) ratio = 2.0-3.0. Protein C and S activity was markedly below (<-20%) and factor VIII was 2- to 11-fold above the human calibration standard, so a standard curve had to be prepared from canine pooled plasma. Reference intervals for protein C, protein S, and factor VIII were 75.5-118.9%, 74.4-160.5%, and 70.9-136.4%, respectively, compared with a canine standard curve. Streptokinase-activated plasminogen assay was not suitable for dogs. There was no significant impact of sex on hemostasis test results. Factor VIII activity, AT, protein C, protein S, and APC ratio were overestimated in hemolytic plasma, whereas fibrinogen, TT, and APTT were underestimated. Lipemia resulted only in false-high D-dimers. This study provided useful reference intervals for dogs, but some human tests (i.e., protein C, protein S, factor VIII, and plasminogen) required modification.
对于STA Compact自动凝血分析仪，犬类凝血参数的参考区间鲜有测定。因此，本研究旨在验证该分析仪的检测方法，并建立其用于犬类样本时的参考范围。对56只健康犬进行了凝血参数评估，这些犬的年龄中位数为2岁，性别分布均匀。95%的参考区间如下：一期凝血酶原时间=5.7 - 8.0秒；活化部分凝血活酶时间（APTT）=10.0 - 14.3秒；凝血酶时间（TT）=11.9 - 18.3秒；纤维蛋白原=1.3 - 3.1克/升；抗凝血酶（AT）=107.9 - 128.0%；D - 二聚体=0.023 - 0.65微克/毫升；抗Xa因子=0.04 - 0.26国际单位/升；活化蛋白C（APC）比值=2.0 - 3.0。蛋白C和S活性明显低于（<-20%）人类校准标准，而因子VIII比人类校准标准高2至11倍，因此必须用犬类混合血浆制备标准曲线。与犬类标准曲线相比，蛋白C、蛋白S和因子VIII的参考区间分别为75.5 - 118.9%、74.4 - 160.5%和70.9 - 136.4%。链激酶激活的纤溶酶原检测不适用于犬类。性别对止血检测结果无显著影响。在溶血血浆中，因子VIII活性、AT、蛋白C、蛋白S和APC比值被高估，而纤维蛋白原、TT和APTT被低估。脂血仅导致D - 二聚体假性升高。本研究为犬类提供了有用的参考区间，但一些人类检测项目（即蛋白C、蛋白S、因子VIII和纤溶酶原）需要进行修改。
