Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People's Republic of China.
Biomed Mater. 2009 Dec;4(6):065005. doi: 10.1088/1748-6041/4/6/065005.
In the world, bone tuberculosis is still very difficult to treat and presents a challenge to clinicians. In this study, we utilized 3D printing technology to fabricate a programmed release multi-drug implant for bone tuberculosis therapy. The construction of the drug implant was a multi-layered concentric cylinder divided into four layers from the center to the periphery. Isoniazid and rifampicin were distributed individually into the different layers in a specific sequence of isoniazid-rifampicin-isoniazid-rifampicin. The drug release assays in vitro and in vivo showed that isoniazid and rifampicin were released orderly from the outside to the center to form the multi-drug therapeutic alliance, and the peak concentrations of drugs were detected in sequence at 8 to 12 day intervals. In addition, no negative effect on the proliferation of rabbit bone marrow mesenchymal stem cells was detected during the cytocompatibility assay. Due to its ideal pharmacologic action and cytocompatibility, the programmed release multi-drug implant with a complex construction fabricated by 3D printing technology could be of interest in prevention and treatment of bone tuberculosis.
在世界范围内，骨结核仍然很难治疗，这对临床医生来说是一个挑战。在本研究中，我们利用 3D 打印技术为骨结核治疗制造了一种编程释放的多药物植入物。药物植入物的构建是一个多层同心圆柱，从中心到外围分为四层。异烟肼和利福平分别以异烟肼-利福平-异烟肼-利福平的特定顺序分布在不同的层中。体外和体内药物释放试验表明，异烟肼和利福平从外到内有序释放，形成多药治疗联盟，药物的峰值浓度以 8 至 12 天的间隔顺序检测到。此外，细胞相容性试验中未检测到对兔骨髓间充质干细胞增殖的负面影响。由于其理想的药理作用和细胞相容性，通过 3D 打印技术制造的具有复杂结构的编程释放多药物植入物可能对预防和治疗骨结核有兴趣。
