Laboratory of Immunology, Environmental Microbiology and Cancerology, Faculty of Sciences Bizerte, Zarzouna, Tunisia.
J Pharm Pharmacol. 2009 Nov;61(11):1545-54. doi: 10.1211/jpp/61.11.0015.
Raphanus sativus (radish) is a species of crucifer, which includes widely consumed vegetables, distributed in Asia, Africa and Europe. It is a rich source of bioactive molecules including anthocyanins, glucosinolates, isothiocyanates and other flavonoids, and miscellaneous phenolic substances. We have evaluated the hepatoprotection of R. sativus extract against zearalenone, an estrogenic mycotoxin initiating hepatotoxicity in male Balb/c mice.
Animals were divided into seven treatment groups and treated orally each day for twenty eight days as follows: a control, an olive oil group, group I, group II, and group III treated with radish extract alone (5, 10 and 15 mg/kg, respectively), group IV treated with zearalenone (40 mg/kg), and group V treated with zearalenone plus the lowest dose of radish extract.
Administration of zearalenone alone resulted in significant decreases in the levels of alkaline phosphatase, lactate dehydrogenase, alanine and aspartate aminotransferases in the liver, suggesting hepatic damage. Moreover, a marked increase in the level of lipid peroxide and concomitant decrease of glutathione peroxidase, glutathione reductase, superoxide dismutase, catalase, glutathione-S-transferase, RNA and DNA concentrations were also observed in the liver tissue of zearalenone-treated mice. Co-treatment with R. sativus extract plus zearalenone succeeded in reversing the condition back to normal levels for all studied parameters.
By itself R. sativus extract did not show any toxic effects and could be considered as a potent hepatoprotectant.
萝卜（radish）是十字花科的一种，包括许多被广泛食用的蔬菜，分布在亚洲、非洲和欧洲。它是生物活性分子的丰富来源，包括花青素、硫代葡萄糖苷、异硫氰酸盐和其他类黄酮以及各种酚类物质。我们评估了萝卜提取物对玉米赤霉烯酮（一种引发雄性 Balb/c 小鼠肝毒性的雌激素真菌毒素）的保肝作用。
动物分为 7 个治疗组，每天口服处理 28 天，如下所示：对照组、橄榄油组、I 组、II 组、III 组分别单独用萝卜提取物（5、10 和 15mg/kg）处理、IV 组用玉米赤霉烯酮（40mg/kg）处理、V 组用玉米赤霉烯酮加最低剂量萝卜提取物处理。
单独给予玉米赤霉烯酮导致肝中碱性磷酸酶、乳酸脱氢酶、丙氨酸和天冬氨酸转氨酶水平显著降低，提示肝损伤。此外，还观察到玉米赤霉烯酮处理小鼠肝组织中脂质过氧化物水平显著升高，同时谷胱甘肽过氧化物酶、谷胱甘肽还原酶、超氧化物歧化酶、过氧化氢酶、谷胱甘肽-S-转移酶、RNA 和 DNA 浓度降低。萝卜提取物与玉米赤霉烯酮共同处理成功地使所有研究参数恢复到正常水平。
萝卜提取物本身没有显示出任何毒性作用，可以被认为是一种有效的保肝剂。
