University of Bristol, Interface Analysis Centre, 121 St Michaels Hill, Bristol, BS2 8 BS, UK.
Phys Med Biol. 2009 Dec 7;54(23):7077-87. doi: 10.1088/0031-9155/54/23/003. Epub 2009 Nov 11.
Raman spectroscopy is a powerful tool for studying biochemical changes in the human body. We describe a miniature, confocal fibre optic probe intended to fit within the instrument channel of a standard medical endoscope. This probe has been optimized for the study of the carcinogenesis process of oesophageal malignancy. The optical design and fabrication of this probe is described including the anisotropic wet etching technique used to make silicon motherboards and jigs. Example spectra of PTFE reference samples are shown. Spectra with acquisition times as low as 2 s from resected oesophageal tissue are presented showing identifiable biochemical changes from various pathologies.
拉曼光谱是研究人体生物化学变化的有力工具。我们描述了一种微型共焦光纤探头，旨在适合标准医用内窥镜的器械通道内。该探头已针对食管恶性肿瘤癌变过程的研究进行了优化。描述了该探头的光学设计和制造，包括用于制作硅母板和夹具的各向异性湿法刻蚀技术。展示了 PTFE 参考样品的示例光谱。从切除的食管组织中获得的采集时间低至 2 秒的光谱显示了各种病变的可识别生物化学变化。
