Hattori Yusuke, Komachi Yuichi, Asakura Toru, Shimosegawa Toru, Kanai Gen-Ichi, Tashiro Hideo, Sato Hidetoshi
Optical Biopsy Development Research Unit, The Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako-shi, 351-0198, Japan.
Appl Spectrosc. 2007 Jun;61(6):579-84. doi: 10.1366/000370207781269747.
A small endoscope system equipped with a micro Raman probe is developed for in vivo Raman measurements in living rats. The measurements are done under anesthesia and artificial respiration to minimize the impact on the rats. Raman spectra of living rat esophagus and stomach are successfully measured. Our results suggest that the Raman spectra reflect subsurface tissue structure that cannot be distinguished in the endoscope image. After the experiments, rats recover without any aftereffects. It is verified that the Raman measurement using the present system is safe and noninvasive for rats.
开发了一种配备微型拉曼探针的小型内窥镜系统，用于对活体大鼠进行体内拉曼测量。测量在麻醉和人工呼吸条件下进行，以尽量减少对大鼠的影响。成功测量了活体大鼠食管和胃的拉曼光谱。我们的结果表明，拉曼光谱反映了在内窥镜图像中无法区分的表面下组织结构。实验后，大鼠恢复且无任何后遗症。证实了使用本系统进行拉曼测量对大鼠是安全且无创的。
