Aspriello Simone Domenico, Zizzi Antonio, Lucarini Guendalina, Rubini Corrado, Faloia Emanuela, Boscaro Marco, Tirabassi Giacomo, Piemontese Matteo
Division of Periodontology, Department of Clinical and Dental Sciences, Polytechnic University of Marche, Torrette, Ancona, Italy.
J Periodontol. 2009 Nov;80(11):1783-9. doi: 10.1902/jop.2009.090239.
Periodontal disease is one of the major problems encountered in patients with diabetes mellitus (DM), and vascular changes may contribute to periodontitis. Our aim was to investigate vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in patients with periodontitis with and without DM.
Immunohistochemical detection of VEGF and MVD analysis, evaluated by CD34+ endothelial cell counts, were performed in 66 gingival samples from patients with generalized, severe, chronic periodontitis who were divided into three groups: 22 participants without systemic diseases (controls), 22 participants with type 1 DM (T1DM), and 22 participants with type 2 DM (T2DM).
In patients with T1DM or T2DM, positive VEGF cells were found to be significantly increased in the epithelium compared to controls. In patients with T1DM, endothelial VEGF expression and MVD were significantly greater than in patients with T2DM and controls.
In patients with diabetes, VEGF overexpression plays a primary role in promoting the extravasation of inflammatory cells, suggesting a useful antiangiogenic strategy for periodontitis treatment. The decreased endothelial VEGF expression and MVD found in patients with T2DM may be caused by insulin resistance and endothelial dysfunction, which are often present in patients with T2DM.
牙周病是糖尿病患者面临的主要问题之一，血管变化可能导致牙周炎。我们的目的是研究伴或不伴糖尿病的牙周炎患者中血管内皮生长因子（VEGF）的表达及微血管密度（MVD）。
对66例患有广泛性、重度、慢性牙周炎的患者的牙龈样本进行VEGF免疫组化检测及通过CD34 + 内皮细胞计数评估的MVD分析，这些患者被分为三组：22例无全身疾病的参与者（对照组），22例1型糖尿病（T1DM）患者，以及22例2型糖尿病（T2DM）患者。
与对照组相比，T1DM或T2DM患者上皮中的VEGF阳性细胞显著增加。T1DM患者的内皮VEGF表达和MVD显著高于T2DM患者和对照组。
在糖尿病患者中，VEGF过表达在促进炎症细胞外渗中起主要作用，提示这是一种治疗牙周炎的有效抗血管生成策略。T2DM患者中内皮VEGF表达和MVD降低可能是由胰岛素抵抗和内皮功能障碍引起的，而这在T2DM患者中很常见。
