Departamento de Biologia Molecular y Biotecnología, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Circuito Escolar s/n, Ciudad Universitaria, Mexico DF, CP 04510, Mexico.
J Clin Virol. 2010 Jan;47(1):43-8. doi: 10.1016/j.jcv.2009.10.008. Epub 2009 Nov 10.
Data on the prevalence of different human papillomavirus (HPV) genotypes and the associated mucosal immune response in women with cervical ectopy are scarce.
To assess the prevalence of different HPV genotypes and the mucosal anti-viral immune response in cervical ectopy.
Detection and typing of HPV DNA was determined in 141 women with cervical ectopy, 272 cytologically normal controls and 98 low-grade squamous intraepithelial lesions (LSIL) by PCR and direct sequencing. Mucosal IgA antibodies to HPV16 and HPV18 were evaluated in cervical mucus by ELISA.
The prevalence of HPV in cervical ectopy was higher (73.7%) than that observed in control samples (30.5% in endocervix, and 1.8% in exocervix), but similar to the prevalence in LSIL (62.2%). Typing showed that the overall distribution frequency concerned 14 different genotypes, with HPV18 being the most prevalent in cervical ectopy (53.9%), whereas HPV16 predominated in LSIL (38.7%). High-risk HPV genotypes were 2.2 times more frequent in cervical ectopy than in the normal endocervix (p<0.0001). HPV infection in cervical ectopy patients was accompanied by a mucosal IgA-antibody response. Antibody reactivity to HPV18 was significantly higher than the response to HPV16.
Cervical ectopy is a risk factor for infection with high-risk HPV genotypes, in particular HPV18. Our results emphasize the need of further studies to clarify the oncogenic potential of this virus in cervical ectopy.
关于宫颈柱状上皮异位患者中不同人乳头瘤病毒（HPV）基因型的流行情况及其相关黏膜免疫应答的数据很少。
评估宫颈柱状上皮异位中不同 HPV 基因型的流行情况和黏膜抗病毒免疫应答。
采用 PCR 和直接测序法，对 141 例宫颈柱状上皮异位患者、272 例细胞学正常对照者和 98 例低度鳞状上皮内病变（LSIL）患者的 HPV DNA 进行检测和分型。采用 ELISA 法检测宫颈黏液中 HPV16 和 HPV18 的黏膜 IgA 抗体。
宫颈柱状上皮异位中的 HPV 患病率（73.7%）高于对照组（宫颈内口为 30.5%，宫颈外口为 1.8%），但与 LSIL 患病率（62.2%）相似。分型显示，涉及 14 种不同基因型，HPV18 是宫颈柱状上皮异位中最常见的基因型（53.9%），而 HPV16 在 LSIL 中占主导地位（38.7%）。高危型 HPV 基因型在宫颈柱状上皮异位中的频率是正常宫颈内口的 2.2 倍（p<0.0001）。宫颈柱状上皮异位患者的 HPV 感染伴有黏膜 IgA 抗体应答。HPV18 抗体反应性明显高于 HPV16。
宫颈柱状上皮异位是感染高危型 HPV 基因型（尤其是 HPV18）的危险因素。我们的研究结果强调需要进一步研究以明确该病毒在宫颈柱状上皮异位中的致癌潜能。
