Naffati Abdulhakim, Mišan Aleksandra, Kenig Saša, Kramberger Katja, Jenko Pražnikar Zala, Petelin Ana, Kljakić Aleksandra Cvetanović, Benmerzoug Abdelmoumen, Elfarrah Nasrien, Stupar Alena
Libyan Medical Research Centre, Az Zawiyah 023-15583, Libya.
Institute of Food Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
Foods. 2025 May 16;14(10):1768. doi: 10.3390/foods14101768.
This study explores the potential of ultrasound-assisted extraction (UAE) for efficiently recovering bioactive compounds, particularly rutin, from the often-overlooked leaves of . By fine-tuning important extraction parameters such as ultrasound power, temperature, and ethanol concentration, this research achieved maximum yields of total phenolic content and rutin, establishing these leaves as a sustainable and valuable source of phytochemicals for applications in functional foods, nutraceuticals, and pharmaceuticals. High-performance liquid chromatography (HPLC) analysis revealed a high rutin content (15.51 mg/g DW), accompanied by considerable amounts of quercetin-3-glucoside and catechin. In vitro assays revealed the extract's strong antioxidative properties, effectively safeguarding Caco-2 and Hep G2 cells against oxidative stress and significantly lowering reactive oxygen species levels. Additionally, the extract enhanced the expression of vital antioxidative enzymes and demonstrated no toxicity at the tested concentrations, confirming its safety for dietary supplement use. These findings underscore the extract's strong safety profile and notable bioefficacy, reinforcing its suitability for incorporation into health-oriented formulations such as functional foods, dietary supplements, or nutraceutical products. Beyond demonstrating biological relevance, this research also advances the sustainable valorization of leaf biomass-an underutilized resource with high phytochemical potential-while contributing to the development of innovative, plant-based strategies for disease prevention and overall health enhancement.
本研究探索了超声辅助提取（UAE）从常被忽视的[植物名称]叶片中高效回收生物活性化合物，特别是芦丁的潜力。通过微调超声功率、温度和乙醇浓度等重要提取参数，本研究实现了总酚含量和芦丁的最大产量，确立了这些叶片是用于功能性食品、营养保健品和药品的可持续且有价值的植物化学物质来源。高效液相色谱（HPLC）分析显示芦丁含量高（15.51毫克/克干重），同时伴有大量的槲皮素 - 3 - 葡萄糖苷和儿茶素。体外试验表明提取物具有很强的抗氧化性能，能有效保护Caco - 2和Hep G2细胞免受氧化应激，并显著降低活性氧水平。此外，提取物增强了重要抗氧化酶的表达，并且在测试浓度下未显示出毒性，证实了其作为膳食补充剂使用的安全性。这些发现强调了提取物强大的安全性和显著的生物功效，加强了其适用于纳入功能性食品、膳食补充剂或营养保健品等以健康为导向的配方中。除了证明生物学相关性外，本研究还推动了[植物名称]叶片生物质这一具有高植物化学潜力但未充分利用的资源的可持续增值，同时有助于开发创新的、基于植物的疾病预防和整体健康促进策略。
