Centers for Disease Control and Prevention, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Division of Viral and Rickettsial Diseases, Poxvirus and Rabies Branch, Atlanta, Georgia 30329, USA.
J Clin Microbiol. 2010 Jan;48(1):268-76. doi: 10.1128/JCM.01697-09. Epub 2009 Nov 11.
Chordopoxviruses of the subfamily Chordopoxvirinae, family Poxviridae, infect vertebrates and consist of at least eight genera with broad host ranges. For most chordopoxviruses, the number of viral genes and their relative order are highly conserved in the central region. The GC content of chordopoxvirus genomes, however, evolved into two distinct types: those with genome GC content of more than 60% and those with a content of less than 40% GC. Two standard PCR assays were developed to identify chordopoxviruses based on whether the target virus has a low or high GC content. In design of the assays, the genus Avipoxvirus, which encodes major rearrangements of gene clusters, was excluded. These pan-pox assays amplify DNA from more than 150 different isolates and strains, including from primary clinical materials, from all seven targeted genera of chordopoxviruses and four unclassified new poxvirus species. The pan-pox assays represent an important advance for the screening and diagnosis of human and animal poxvirus infections, and the technology used is accessible to many laboratories worldwide.
痘病毒科（Poxviridae）的痘病毒亚科（Chordopoxvirinae）的疱疹病毒感染脊椎动物，至少包含 8 个具有广泛宿主范围的属。对于大多数疱疹病毒而言，中央区域的病毒基因数量及其相对顺序高度保守。然而，疱疹病毒基因组的 GC 含量已经进化为两种截然不同的类型：GC 含量超过 60%和低于 40%的基因组。根据目标病毒的 GC 含量高低，开发了两种标准的 PCR 检测方法来鉴定疱疹病毒。在设计这些检测方法时，排除了编码基因簇主要重排的禽痘病毒属（Avipoxvirus）。这些泛痘病毒检测方法可扩增来自 150 多种不同分离株和菌株的 DNA，包括来自原发性临床材料、所有 7 种靶向疱疹病毒属和 4 种未分类的新痘病毒种的 DNA。泛痘病毒检测方法是筛查和诊断人类和动物痘病毒感染的重要进展，并且该技术在全球范围内为许多实验室所掌握。
