Fei Yan, Lu Guocai, Fan Guorong, Wu Yutian
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, P.R. China.
Anal Sci. 2009 Nov;25(11):1333-8. doi: 10.2116/analsci.25.1333.
The binding of sinafloxacin to DNA has been studied by fluorescence spectroscopy and UV-vis absorption spectroscopy. The variations in the spectroscopic characteristics of sinafloxacin upon addition of DNA were observed. Salt concentration effect, KI quenching, fluorescence polarization, DNA denaturation experiments, melting temperature (T(m)) curves and viscosity measurements were studied to investigate the binding mechanism. It was proved that the fluorescence quenching of sinafloxacin is a result of the formation of sinafloxacin-DNA complex. Quenching constants were determined using the Stern-Volmer equation. From the experiment evidence, the major binding mode of sinafloxacin with DNA was evaluated to be the groove binding.
通过荧光光谱法和紫外可见吸收光谱法研究了西那沙星与DNA的结合。观察了加入DNA后西那沙星光谱特性的变化。研究了盐浓度效应、KI猝灭、荧光偏振、DNA变性实验、熔解温度（T(m)）曲线和粘度测量，以研究结合机制。结果表明，西那沙星的荧光猝灭是西那沙星-DNA复合物形成的结果。使用Stern-Volmer方程测定猝灭常数。根据实验证据，评估西那沙星与DNA的主要结合模式为沟槽结合。
