GMCA Research Group, Departamento de Microbiología y Ecología, Facultad de Farmacia, Universidad de Valencia, Avenida Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
Yeast. 2010 Feb;27(2):77-87. doi: 10.1002/yea.1733.
The Cwt1 transcription factor is involved in cell wall architecture of the human fungal pathogen Candida albicans. We demonstrate here that deficiency of Cwt1 leads to decreased beta1,6-glucan in the cell wall, while mannoproteins are increased in the cell wall of exponentially growing cells and are released into the medium of stationary phase cells. Hyphal morphogenesis of cwt1 mutants is reduced on the surfaces of some inducing media. Unexpectedly, the CWT1/cwt1 heterozygous strains shows some stronger in vitro phenotypes compared to the homozygous mutant. The heterozygous but not the homozygous strain is also strongly impaired for its virulence in a mouse model of systemic infection. We suggest that an intermediate dosage of Cwt1 affects phenotypes profoundly, while its complete absence may elicit compensatory responses of C. albicans.
Cwt1 转录因子参与了人类真菌病原体白色念珠菌的细胞壁结构。我们在这里证明，Cwt1 的缺乏导致细胞壁中β1,6-葡聚糖减少，而甘露糖蛋白在指数生长期细胞的细胞壁中增加，并释放到静止期细胞的培养基中。在一些诱导培养基的表面，cwt1 突变体的菌丝形态发生减少。出乎意料的是，与纯合突变体相比，CWT1/cwt1 杂合株系在体外表型上表现出一些更强的表型。杂合但非纯合菌株在系统性感染的小鼠模型中也严重削弱了其毒力。我们认为，Cwt1 的中等剂量会深刻影响表型，而其完全缺失可能会引发白色念珠菌的代偿反应。
