Lei Yun, Tang Haiyang, Feng Meifu, Zou Bingsuo
School of Resource and Environmental Engineering, Wuhan University of Technology, Wuhan, Hubei 430070, China.
J Nanosci Nanotechnol. 2009 Oct;9(10):5726-30. doi: 10.1166/jnn.2009.1258.
Fluorescent quantum dots have great potential to act as labels in biological research, especially cellular tracking in vivo. Here, small thiol molecules-capped CdSe/ZnS quantum dots attached to Tat peptide were used for label agents, and introduced into living stem cells. The stem cells labeled with quantum dots were intravenously injected into the tail veins of NOD/SCID beta2M null mice, and whose tissue sections of major organs of null mice were examined with fluorescence microscope to assess the distribution of transplanted stem cells. Stem cells with internalized quantum dots offer a promising approach in stem cell transplantation, which will hold a significant impact on stem cell based therapy for several disorders.
荧光量子点在生物学研究中作为标记物具有巨大潜力，尤其是在体内细胞追踪方面。在此，将与Tat肽相连的小分子硫醇修饰的CdSe/ZnS量子点用作标记剂，并导入活的干细胞中。将用量子点标记的干细胞静脉注射到NOD/SCID β2M基因敲除小鼠的尾静脉中，然后用荧光显微镜检查基因敲除小鼠主要器官的组织切片，以评估移植干细胞的分布。内化量子点的干细胞为干细胞移植提供了一种有前景的方法，这将对基于干细胞的多种疾病治疗产生重大影响。
