Lei Yun, Tang Haiyang, Zhong Haizheng, Xie Haiyan, Liu Ruibin, Feng Meifu, Zou Bingsuo
Micro Nano Technology Center, Beijing Institute of Technology, Beijing 100081, China.
J Nanosci Nanotechnol. 2012 Sep;12(9):6880-6. doi: 10.1166/jnn.2012.5855.
Fluorescent quantum dots have great potential to act as labeling tools in biological research, especially cellular tracking and imaging. Tat peptide conjugated quantum dots were introduced into living human hematopoietic stem cells (HSCs). The internalized quantum dots were assessed by laser confocal microscope and flow cytometer. The quantum dots labeled HSCs were injected intravenously into the tail veins of NOD/SCID beta2M null mice. The tissue collections in the major organs were examined with fluorescence microscope to assess the distribution of transplanted stem cells. HSCs with internalized quantum dots offer a promising approach for stem cell transplantation, which will hold a significant impact on stem cell based therapy for several disorders, especially to cure leukemia in current China.
荧光量子点在生物研究中作为标记工具具有巨大潜力，尤其是在细胞追踪和成像方面。将与Tat肽偶联的量子点导入人类活造血干细胞（HSCs）。通过激光共聚焦显微镜和流式细胞仪评估内化的量子点。将量子点标记的造血干细胞静脉注射到NOD/SCID β2M基因敲除小鼠的尾静脉中。用荧光显微镜检查主要器官中的组织样本，以评估移植干细胞的分布。内化量子点的造血干细胞为干细胞移植提供了一种有前景的方法，这将对基于干细胞的多种疾病治疗产生重大影响，尤其是在当前中国治疗白血病方面。
